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首页> 外文期刊>Archives of otolaryngology--head & neck surgery. >Time-resolved fluorescence spectroscopy as a diagnostic technique of oral carcinoma: Validation in the hamster buccal pouch model.
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Time-resolved fluorescence spectroscopy as a diagnostic technique of oral carcinoma: Validation in the hamster buccal pouch model.

机译：时间分辨荧光光谱法作为口腔癌的诊断技术：在仓鼠颊囊模型中的验证。

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OBJECTIVE: To investigate the benefit of using time-resolved, laser-induced fluorescence spectroscopy for diagnosing malignant and premalignant lesions of the oral cavity. DESIGN: The carcinogen 7,12-dimethylbenz[a]anthracene (DMBA) was applied to 1 cheek pouch of 19 hamsters. The contralateral pouch and the cheek pouches of 3 hamsters without DMBA exposure served as controls. SETTING: University of California, Davis. PARTICIPANTS: Twenty-two golden/Syrian hamsters. INTERVENTION: A nitrogen pulse laser was used to induce tissue autofluorescence between the wavelengths of 360 and 650 nm. MAIN OUTCOME MEASURES: Spectral intensities and time-domain measurements were obtained and compared with the histopathologic findings at each corresponding site. RESULTS: Spectral intensities and lifetime values at 3 spectral bands (SBs; SB1 = 380 +/- 10 nm; SB2 = 460 +/- 10 nm, and SB3 = 635 +/- 10 nm) allowed for discrimination among healthy epithelium, dysplasia, carcinoma in situ, and invasive carcinoma. The lifetime values at SB2 were the most important when distinguishing the lesions using only time-resolved parameters. An algorithm combining spectral fluorescence parameters derived from both spectral and time-domain parameters (peak intensities, average fluorescence lifetimes, and the Laguerre coefficient [zero-order]) for healthy epithelium, dysplasia, carcinoma in situ, and invasive carcinoma provided the best diagnostic discrimination, with 100%, 100%, 69.2%, and 76.5% sensitivity and 100%, 92.2%, 97.1%, and 96.2% specificity, respectively. CONCLUSIONS: The addition of time-resolved fluorescence-derived parameters significantly improves the capability of fluorescence spectroscopy-based diagnostics in the hamster buccal pouch. This technique provides a potential noninvasive diagnostic instrument for head and neck cancer.
机译：目的：探讨使用时间分辨的激光诱导荧光光谱法诊断口腔恶性和癌前病变的益处。设计：将致癌物7,12-二甲基苯并[a]蒽（DMBA）应用于19只仓鼠的1个面颊袋。将没有暴露于DMBA的3只仓鼠的对侧小袋和脸颊小袋作为对照。地点：加州大学戴维斯分校。参与者：22只金色/叙利亚仓鼠。干预：使用氮气脉冲激光在360至650 nm波长之间诱导组织自发荧光。主要观察指标：获得光谱强度和时域测量值，并将其与每个相应部位的组织病理学结果进行比较。结果：在3个光谱带（SBs; SB1 = 380 +/- 10 nm; SB2 = 460 +/- 10 nm; SB3 = 635 +/- 10 nm）的光谱强度和寿命值可区分健康上皮，异型增生，原位癌和浸润性癌。仅使用时间分辨的参数区分病变时，SB2的生命值最为重要。结合从光谱和时域参数（峰值强度，平均荧光寿命和Laguerre系数[零级]）得出的光谱荧光参数的算法，对于健康的上皮，异型增生，原位癌和浸润性癌提供了最佳诊断分别具有100％，100％，69.2％和76.5％的敏感性和100％，92.2％，97.1％和96.2％的特异性。结论：时间分辨的荧光派生参数的添加大大提高了基于荧光光谱的仓鼠颊囊诊断的能力。该技术为头颈癌提供了一种潜在的非侵入性诊断工具。

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