• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Archives of microbiology >In silico and wet-lab study revealed cadmium is the potent inhibitor of HupL in Anabaena sp PC C 7120
【24h】

In silico and wet-lab study revealed cadmium is the potent inhibitor of HupL in Anabaena sp PC C 7120

机译：在计算机和湿实验室研究中，发现镉是鱼腥藻PC C 7120中HupL的有效抑制剂。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The hupL of Anabaena sp. PCC 7120 encodes the large subunit of uptake hydrogenase found in all diazotrophic cyanobacteria and boosts up the nitrogen-fixing potential by catalyzing the removal of the molecular hydrogen produced as a by-product of dinitrogen fixation. Bioinformatics analysis revealed that HupL from Anabaena sp. PCC7120 is a 60.2 kDa, thermostable, glycine-rich protein having highest structural similarity with NiFeSe hydrogenase of Desulfomicrobium baculatumis. Toxicity of selected abiotic stresses like arsenic, cadmium, copper, and salt with HupL was further reconciled by wet-lab approaches like qRT-PCR, hydrogenase and nitrogenase activity assay as hydrogenases unintendedly affect the nitrogenase activity in Anabaena. Down-regulated transcript along with highly inhibited hydrogenase and nitrogenase activities under cadmium stress revealed that cadmium is a potent inhibitor of hydrogenases in Anabaena which indirectly affects its nitrogen-fixing capabilities.
机译：鱼腥藻的种。 PCC 7120编码在所有重氮蓝藻细菌中发现的摄取氢化酶的大亚基，并通过催化去除作为固氮副产物而产生的分子氢，提高固氮潜力。生物信息学分析表明，来自鱼腥藻的HupL。 PCC7120是一种60.2 kDa，热稳定，富含甘氨酸的蛋白质，与杆状脱硫微球菌的NiFeSe氢化酶具有最高的结构相似性。选择性的非生物胁迫（如砷，镉，铜和盐）对HupL的毒性通过湿实验室方法（如qRT-PCR，氢化酶和固氮酶活性测定法）进一步得到了调节，因为氢化酶意外地影响了鱼腥藻中的固氮酶活性。转录下调以及在镉胁迫下高度抑制的氢酶和固氮酶活性表明，镉是鱼腥藻中强力的氢酶抑制剂，间接影响其固氮能力。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号