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首页> 外文期刊>Archives of virology >Phylogenetic analysis of porcine epidemic diarrhea virus (PEDV) field strains in central China based on the ORF3 gene and the main neutralization epitopes
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Phylogenetic analysis of porcine epidemic diarrhea virus (PEDV) field strains in central China based on the ORF3 gene and the main neutralization epitopes

机译：基于ORF3基因和主要中和表位的华中地区猪流行性腹泻病毒（PEDV）野外菌株的系统发育分析

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Since 2010, porcine epidemic diarrhea has re-emerged with devastating impact on the swine-raising industry in central China. To investigate the epidemic characteristics of PEDV, the complete ORF3 genes of 14 PEDV field strains from central China during 2012 to 2013 were cloned, sequenced and compared with reference strains. Phylogenetic analysis based on the complete ORF3 gene showed that the PEDVs in central China and the reference strains could be divided into three groups: G1, G2, and G3. The 14 PEDV isolates were classified as G1 and showed a close relationship to some Chinese strains isolated previously in central China and differed genetically from recent isolates from southern China, Korean strains (SM98 and DB1865, 2012), the Chinese LZC strain (2007), and the vaccine strain (CV777) being used in China. Our findings suggested that the PEDVs circulating between 2012 and 2013 in central China might have evolved from earlier strains in the local region. To determine the reason for recent vaccination failures, we also studied variations in antigenicity of field strains by analyzing the three neutralizing epitope regions in the S gene. The results showed that the neutralizing epitopes at aa 245-252 were highly conserved, but most of the amino acid changes occurred in the epitope regions aa 7-146 and 271-278. We speculate that the amino acid mutations in the neutralizing epitope regions may be associated with changes in the antigenicity of PEDV and consequently result in vaccination failure. Together, these findings may be useful for understanding the epidemiology of PEDV and may be relevant for designing of new and more efficacious vaccines.
机译：自2010年以来，猪流行性腹泻再次出现，对中国中部养猪业产生了毁灭性影响。为了调查PEDV的流行特征，我们克隆了2012年至2013年间华中地区14株PEDV野外菌株的完整ORF3基因，进行了测序并与参考菌株进行了比较。基于完整ORF3基因的系统进化分析表明，中国中部的PEDV和参考菌株可分为三类：G1，G2和G3。这14个PEDV分离株被分类为G1，与以前在华中地区分离的一些中国菌株显示出密切的关系，并且与最近从中国南方分离的韩国菌株，韩国菌株（SM98和DB1865，2012），中国LZC菌株（2007），以及在中国使用的疫苗株（CV777）。我们的研究结果表明，2012年至2013年间在中国中部传播的PEDV可能是由当地的早期菌株演变而来。为了确定近期疫苗接种失败的原因，我们还通过分析S基因中的三个中和表位区域来研究田间菌株抗原性的变化。结果显示，在氨基酸245-252处的中和表位是高度保守的，但是大多数氨基酸变化发生在氨基酸表位区域aa 7-146和271-278上。我们推测中和性抗原决定簇区域中的氨基酸突变可能与PEDV抗原性的变化有关，因此导致疫苗接种失败。总之，这些发现可能有助于理解PEDV的流行病学，并且可能与设计新的更有效的疫苗有关。

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