...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Archives of Toxicology >Gene expression profiling in lung tissues from rats exposed to formaldehyde.
【24h】

Gene expression profiling in lung tissues from rats exposed to formaldehyde.

机译：暴露于甲醛的大鼠肺组织中的基因表达谱。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Formaldehyde is a ubiquitous toxic organic compound recently classified as a carcinogen by the International Agency for Research on Cancer and one of the major factors causing sick building syndrome. In this study, we have investigated the effects of formaldehyde on mRNA expression in rat lung tissues by applying genomics. Rats were exposed to ambient air and two different concentrations of formaldehyde (0, 5, 10 ppm) for 2 weeks at 6 h/day and 5 days/week in an inhalation chamber. Malondialdehyde (MDA) assay and carbonyl spectrometric assay were conducted to determine lipid peroxidation and protein oxidation levels and Comet assays were used for genotoxicity evaluation. Level of MDA, carbonyl insertion and DNA damage in the lungs of rats exposed to FA were found to be dose dependently increased. Gene expression was evaluated by using a bio-array hybridization analysis. A total of 21 (2 up- and 19 down-regulated) genes were identified as biomarkers for formaldehyde effects. Several differentiated gene groups were found. Genes involved in apoptosis, immunity, metabolism, signal transduction, transportation, coagulation and oncogenesis were found to be up- and down-regulated. Among these genes, the mRNA expressions of cytochrome P450, hydroxymethylbilane synthase, glutathione reductase, carbonic anhydrase 2, natriuretic peptide receptor 3, lysosomal associated protein transmembrane 5, regulator of G-protein signaling 3, olfactomedin related ER localized protein, and poly (ADP-ribose) polymerase-1 were confirmed by quantitative RT-PCR analysis. In summary, the MDA lipid peroxidation and the carbonyl protein oxidation assays showed that cytotoxic effects increased with increasing formaldehyde levels. Genomic analysis showed that 21 genes were up- or down-regulated. Of these genes, nine were confirmed by quantitative RT-PCR and could be potential biomarkers for human diseases associated with formaldehyde exposure.
机译：甲醛是一种无处不在的有毒有机化合物，最近被国际癌症研究机构归类为致癌物质，并且是导致建筑物综合症的主要因素之一。在这项研究中，我们已经通过应用基因组学研究了甲醛对大鼠肺组织中mRNA表达的影响。将大鼠在吸入室内暴露于环境空气和两种不同浓度的甲醛（0、5、10 ppm）中，分别为6小时/天和5天/周，持续2周。进行了丙二醛（MDA）测定和羰基光谱测定以确定脂质过氧化和蛋白质氧化水平，彗星测定用于遗传毒性评估。发现暴露于FA的大鼠肺中的MDA水平，羰基插入和DNA损伤呈剂量依赖性增加。通过使用生物阵列杂交分析评估基因表达。共有21个基因（2个上调和19个下调）被确定为甲醛影响的生物标记。发现了几个分化的基因组。发现涉及凋亡，免疫，代谢，信号转导，转运，凝血和肿瘤发生的基因被上调和下调。在这些基因中，细胞色素P450，羟甲基胆碱合酶，谷胱甘肽还原酶，碳酸酐酶2，利钠肽受体3，溶酶体相关蛋白跨膜5，G蛋白信号调节剂3，与脂蛋白相关的ER定位蛋白和poly（ADP）的mRNA表达-核糖）聚合酶-1通过定量RT-PCR分析确认。总而言之，MDA脂质过氧化和羰基蛋白氧化试验表明，随着甲醛水平的升高，细胞毒性作用增强。基因组分析表明21个基因被上调或下调。在这些基因中，有9个通过定量RT-PCR证实，可能是与甲醛暴露相关的人类疾病的潜在生物标记。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号