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首页> 外文期刊>Archives of Toxicology >Differential stability of lead sulfide nanoparticles influences biological responses in embryonic zebrafish.
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Differential stability of lead sulfide nanoparticles influences biological responses in embryonic zebrafish.

机译：硫化铅纳米颗粒的差异稳定性影响胚胎斑马鱼的生物学反应。

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摘要

As the number of nanoparticle-based products increase in the marketplace, there will be increased potential for human exposures to these engineered materials throughout the product life cycle. We currently lack sufficient data to understand or predict the inherent nanomaterial characteristics that drive nanomaterial-biological interactions and responses. In this study, we utilized the embryonic zebrafish (Danio rerio) model to investigate the importance of nanoparticle (NP) surface functionalization, in particular as it pertains to nanoparticle stability, on in vivo biological responses. This is a comparative study where two lead sulfide nanoparticles (PbS-NPs) with nearly identical core sizes, but functionalized with either sodium 3-mercaptopropanesulfonate (MT) or sodium 2,3-dimercaptopropanesulfonate (DT) ligand, were used. Developmental exposures and assessments revealed differential biological responses to these engineered nanoparticles. Exposures beginning at 6 h post fertilization (hpf) to MT-functionalized nanoparticles (PbS-MT) led to 100% mortality by 120 hpf while exposure to DT-functionalized nanoparticles (PbS-DT) produced less than a 5% incident in mortality at the same concentration. Exposure to the MT and DT ligands themselves did not produce adverse developmental effects when not coupled to the NP core. Following exposure, we confirmed that the embryos took up both PbS-MT and PbS-DT material using inductively coupled plasma-mass spectrometry (ICP-MS). The stability of the nanoparticles in the aqueous solution was also characterized. The nanoparticles decompose and precipitate upon exposure to air. Soluble lead ions were observed following nanoparticle precipitation and in greater concentration for the PbS-MT sample compared to the PbS-DT sample. These studies demonstrate that in vivo assessments can be effectively used to characterize the role of NP surface functionalization in predicting biological responses.
机译：随着市场上基于纳米颗粒的产品数量的增加，人类在整个产品生命周期中接触这些工程材料的可能性将会增加。我们目前缺乏足够的数据来理解或预测驱动纳米材料-生物相互作用和响应的固有纳米材料特性。在这项研究中，我们利用胚胎斑马鱼（Danio rerio）模型来研究纳米粒子（NP）表面功能化的重要性，尤其是与纳米粒子稳定性有关的生物体内反应。这是一个比较研究，其中使用了两个具有几乎相同核心尺寸，但被3-巯基丙烷磺酸钠（MT）或2,3-二巯基丙烷磺酸钠（DT）配体官能化的硫化铅纳米颗粒（PbS-NPs）。发育过程中的暴露和评估显示出对这些工程化纳米颗粒的不同生物学反应。受精（hpf）在6 h后开始暴露于MT-官能化的纳米颗粒（PbS-MT）导致100％的死亡率，而暴露于DT-官能化的纳米颗粒（PbS-DT）造成的死亡率不到5％。同样的浓度。如果未与NP核心偶联，则MT和DT配体本身的暴露不会产生不利的发育影响。暴露后，我们证实了使用电感耦合等离子体质谱法（ICP-MS）的胚胎吸收了PbS-MT和PbS-DT材料。还表征了纳米颗粒在水溶液中的稳定性。纳米颗粒在暴露于空气时分解并沉淀。与PbS-DT样品相比，在纳米颗粒沉淀后观察到可溶性铅离子，并且PbS-MT样品的浓度更高。这些研究表明，体内评估可以有效地用于表征NP表面功能化在预测生物学反应中的作用。

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