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首页> 外文期刊>Breeding science >Transgenic potato overexpressing Arabidopsis cytosolic AtDHAR1 showed higher tolerance to herbicide, drought and salt stresses
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Transgenic potato overexpressing Arabidopsis cytosolic AtDHAR1 showed higher tolerance to herbicide, drought and salt stresses

机译：过表达拟南芥胞质AtDHAR1的转基因马铃薯对除草剂，干旱和盐胁迫表现出更高的耐受性

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摘要

Ascorbate (vitamin C) is a powerful antioxidant and scavenger of free radicals that protects plants against oxidative damage caused by adverse environmental conditions such as drought, salt stress, and herbicide use. Dehydroascorbate reductase (DHAR; EC 1.8.5.1) and monodehydroascorbate reductase (MDAR; EC 1.6.5.4) are crucial for ascorbate regeneration and the maintenance of a pool of the reduced form. In this study, we report the development of transgenic potato (Solarium tuberosum L.) that overexpresses the Arabidopsis thaliana DHAR gene (AtDHAR1) in the cytosol. The transgenic plants exhibited up to 4.5 times the DHAR activity and up to 2.8 times the level of reduced ascorbate found in the wild-type plants. When subjected to methylviologen treatment, the transgenic plants exhibited enhanced tolerance in terms of less ion leakage, greater chlorophyll contents, less accumulation of hydrogen peroxide, and less severe visual injury symptoms. Moreover, the transgenic plants exhibited faster growth under drought and salt stress. Our results demonstrate that elevating ascorbate contents by overproducing DHAR represents a viable approach for the development of herbicide-tolerant potato.
机译：抗坏血酸（维生素C）是一种强大的抗氧化剂和自由基清除剂，可保护植物免受因干旱，盐胁迫和使用除草剂等不利环境条件而引起的氧化损害。脱氢抗坏血酸还原酶（DHAR; EC 1.8.5.1）和单脱氢抗坏血酸还原酶（MDAR; EC 1.6.5.4）对于抗坏血酸的再生和维持还原形式库至关重要。在这项研究中，我们报告了在细胞质中过表达拟南芥DHAR基因（AtDHAR1）的转基因马铃薯（Solarium tuberosum L.）的发展。转基因植物显示出高达DHAR活性的4.5倍，是野生型植物中抗坏血酸还原水平的2.8倍。当进行甲基紫精处理时，转基因植物表现出更高的耐受性，包括更少的离子泄漏，更大的叶绿素含量，更少的过氧化氢积聚和更少的严重视觉损伤症状。此外，转基因植物在干旱和盐胁迫下表现出更快的生长。我们的结果表明，通过过量生产DHAR来提高抗坏血酸含量代表了开发耐除草剂马铃薯的可行方法。

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