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首页> 外文期刊>Asian Journal of Chemistry: An International Quarterly Research Journal of Chemistry >Extraction and HPLC Analysis of Tanshinone I,Tanshinone IIA and Cryptotanshinone from Salvia miltiorrhiza Bunge
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Extraction and HPLC Analysis of Tanshinone I,Tanshinone IIA and Cryptotanshinone from Salvia miltiorrhiza Bunge

机译:丹参中丹参酮I,丹参酮IIA和隐丹参酮的提取及HPLC分析

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摘要

In the present work,simultaneous extraction and separation of tanshinone I,tanshinone IIA and cryptotanshinone has been developed and validated from Salvia miltiorrhiza bunge. The extracts of the tanshinones were separated on a C_(18) column with the mobile phase composed of methanol-water (78:22,v/v,containing 0.5 % v acetic acid) at a flow-rate of 0.5 mL/min. The calibration was linear in a range of 0.1 -500.0 μg/mL for these three compounds. The extracted amounts are 0.0091 mg/g for the tanshinone 1,0.12 mg/g for the tanshinone IIA and 0.15 mg/g for the cryptotanshinone.
机译:在目前的工作中,丹参酮I,丹参酮IIA和隐丹参酮的同时提取和分离已从丹参中得到了开发和验证。丹参酮的提取物在C_(18)色谱柱上分离,流动相由甲醇-水(78:22,v / v,包含0.5%v的乙酸)组成,流速为0.5 mL / min。对于这三种化合物,校准范围在0.1 -500.0μg/ mL范围内是线性的。丹参酮的提取量为0.0091 mg / g,丹参酮IIA的提取量为1,0.12 mg / g,隐丹参酮的为0.15 mg / g。

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