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首页> 外文期刊>Biochimica et biophysica acta: international journal of biochemistry and biophysics >Purification and characterization of an endo-exonuclease from Podospora anserina mitochondria.
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Purification and characterization of an endo-exonuclease from Podospora anserina mitochondria.

机译：an藜线粒体线粒体内切核酸酶的纯化和鉴定。

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The senescence phenotype of Podospora anserina wild-type strains depends on mitochondrial (mt) genome stability. Characterization of activities implicated in the maintenance of the mt DNA is therefore essential for a better understanding of these degenerative processes. To address this question we looked for a nuclease activity in this fungal mitochondria. Here we describe the purification of an endo-exonuclease active on single-stranded, double-stranded and flap DNA. The Podospora nuclease also possesses an RNase H activity. Gel filtration chromatography showed a native molecular mass of 90 kDa for the P. anserina enzyme. The highly purified fraction shows a single polypeptide chain of 49 kDa on SDS-PAGE, indicating that the Podospora enzyme is probably active as a dimer. Purification and sequencing of the endolysine digestion peptides of the Podospora mt nuclease suggested that this enzyme could belong to the 5' structure-specific endo-exonuclease family. The possible involvement of this nuclease in mt DNA recombination during the senescence process is evoked.
机译：do假单胞菌野生型菌株的衰老表型取决于线粒体（mt）基因组稳定性。因此，对维持mt DNA的活性进行表征对于更好地理解这些变性过程至关重要。为了解决这个问题，我们寻找了这种真菌线粒体中的核酸酶活性。在这里，我们描述了对单链，双链和皮瓣DNA具有活性的内切核酸外切酶的纯化。 do孢核酸酶也具有RNase H活性。凝胶过滤色谱法显示an假单胞菌酶的天然分子量为90 kDa。高度纯化的馏分在SDS-PAGE上显示一条49 kDa的多肽链，表明Podospora酶可能具有二聚体活性。 Podospora mt核酸酶的赖氨酸消化肽的纯化和测序表明，该酶可能属于5'结构特异性核酸内切酶家族。在衰老过程中，这种核酸酶可能参与了mt DNA的重组。

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