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首页> 外文期刊>Artificial cells, blood substitutes, and biotechnology >A new and preliminary three-dimensional perspective: proteomes of optimization between OSCC and OLK.
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A new and preliminary three-dimensional perspective: proteomes of optimization between OSCC and OLK.

机译：新的和初步的三维视角：OSCC和OLK之间的优化蛋白质组。

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BACKGROUND: principally 80 percent of the malignant oral tumors are the Oral Squamous Cell Carcinoma (OSCC), which require quantities of such sacrifices as deformity, malfunction, recurrence, metastasis, deterioration, and mortality in common cases of failing to antedate diagnosis. Similarly critical is the Oral Leukoplakia (OLK) among precancerous lesions of oral mucosa. It would also be of interest for scholars and clinicians to target the discrimination as seizing up OLK intimate to OSCC. OBJECTIVE: through bioinformatics technology, the research in narration worked to establish a three-dimensional discriminate database from high throughput data of protein fingerprints from serum, saliva, and tissue samples of OSCC and OLK patients as a preliminary step towards integrated group proteins biomarker discovery and to further understanding of corresponding tumorgenesis and proteomics. METHODS: differential proteomic patterns in serum, saliva, and tissue between OSCC patients or OSCC tissues and OLK were detected by SELDI-TOF-MS technology, and discriminatively analyzed by ZUCI-PDAS (Zhejiang University Cancer Institute ProteinChip Data Analysis System) with Support Vector Machines (SVM) and cross validation. Additionally, Laser Capture Micro-dissection technology was utilized in the tissue research. RESULTS: mass/charge proteomes of optimization obtained from the samples were, respectively, 4162 with 6886 of 87.82%, 92.86%, 66.67% as the sensitivity, specificity, and accuracy for serum difference; m/z 5818, 4617 with 3884 of all 100% as the sensitivity, specificity, and accuracy for saliva difference; and m/z 3738, 11366 of 96.29%, 100.00%, 85.71% as the sensitivity, specificity, and accuracy for tissue difference between OSCC and OLK patients. CONCLUSIONS: within the fields of clinical biomarker application and bioinformatics utilization, as well as the exploitation and popularization of modern discriminate analysis technology, to determine preventative and therapeutic stage and prognosis of OSCC and OLK, the proteomes of optimization for discrepancy are suggested to be directly enrolled in clinical application without protein identification.
机译：背景：口腔鳞状细胞癌（OSCC）主要是80％的恶性口腔肿瘤，在无法进行早期诊断的常见情况下，它们需要付出一定数量的牺牲，例如畸形，功能失调，复发，转移，恶化和死亡。同样重要的是口腔粘膜癌前病变之间的口腔白斑（OLK）。对于学者和临床医生而言，将歧视定位为紧贴与OSCC密切相关的OLK也会引起人们的兴趣。目的：通过生物信息学技术，叙事研究致力于建立高区分度OSSC和OLK患者血清，唾液和组织样本中蛋白质指纹的高通量数据的三维区分数据库，这是朝着综合性组蛋白质生物标记发现和发展的第一步。进一步了解相应的肿瘤发生和蛋白质组学。方法：采用SELDI-TOF-MS技术检测OSCC患者或OSCC组织与OLK之间血清，唾液和组织中的蛋白质组差异性，并通过支持载体ZUCI-PDAS（浙江大学癌症研究所蛋白质芯片数据分析系统）进行判别分析。机器（SVM）和交叉验证。另外，激光捕获显微解剖技术被用于组织研究。结果：从样品中获得的优化质荷比蛋白质组分别为4162和6886，血清差异的敏感性，特异性和准确性分别为87.82％，92.86％，66.67％。 m / z 5818、4617和3884（共100％）作为唾液差异的敏感性，特异性和准确性;和m / z 3738、11366，分别是OSCC和OLK患者之间组织差异的敏感性，特异性和准确性的96.29％，100.00％，85.71％。结论：在临床生物标志物应用和生物信息学利用以及现代判别分析技术的开发和普及领域，确定OSCC和OLK的预防和治疗阶段以及预后，建议蛋白质组学的差异最直接参加临床应用但未鉴定蛋白质。

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