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首页> 外文期刊>Biochimica et biophysica acta: international journal of biochemistry and biophysics >Retrograde transport of protein toxins under conditions of COPI dysfunction.
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Retrograde transport of protein toxins under conditions of COPI dysfunction.

机译：在COPI功能障碍的条件下逆行转运蛋白毒素。

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摘要

Retrograde transport dependent on coat protein I (COPI) was impaired using two different approaches and the effects on the retrograde transport of protein toxins were investigated. One approach was to study ldlF cells that express a temperature-sensitive defect in the epsilon-COP subunit of COPI. The second approach was to treat cells with 1,3-cyclohexanebis(methylamine) (CBM), a drug that interferes with the binding of COPI to Golgi membranes. With both approaches, cells remained sensitive to a variety of protein toxins regardless of whether the toxins contained a KDEL motif. Moreover, cholera toxin, which contains a KDEL sequence, was observed by immunofluorescence microscopy to enter the endoplasmic reticulum of Vero cells in the presence of CBM. These data support published evidence indicating the presence in cells of a COPI- and KDEL receptor-independent pathway of retrograde transport from the Golgi complex to the endoplasmic reticulum. In addition, the results suggest that certain toxins containing a KDEL motif may use either the COPI-dependent or COPI-independent pathway of retrograde transport.
机译：逆转转运依赖外壳蛋白I（COPI）受到损害，使用两种不同的方法，并研究了对蛋白质毒素逆行转运的影响。一种方法是研究在COPI的ε-COP亚基中表达温度敏感缺陷的ldlF细胞。第二种方法是用1,3-环己烷双（甲胺）（CBM）处理细胞，该药物会干扰COPI与高尔基体膜的结合。两种方法都可以使细胞对多种蛋白质毒素保持敏感，而无论这些毒素是否包含KDEL基序。此外，在存在CBM的情况下，通过免疫荧光显微镜观察到含有KDEL序列的霍乱毒素进入了Vero细胞的内质网。这些数据支持已发表的证据，表明细胞中存在从高尔基复合体到内质网的逆行转运的COPI和KDEL受体非依赖性途径。此外，结果表明某些含有KDEL基序的毒素可能使用了逆行转运的COPI依赖性或COPI依赖性途径。

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