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首页> 外文期刊>Artificial cells, blood substitutes, and biotechnology >Effect of polymer concentration on camouflaging of pancreatic islets with mPEG-succinimidyl carbonate
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Effect of polymer concentration on camouflaging of pancreatic islets with mPEG-succinimidyl carbonate

机译：聚合物浓度对mPEG-琥珀酰亚胺碳酸酯对胰岛伪装的影响

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摘要

Modifying the surface of living cells with specially selected polymers is a new method of protecting them from the immune system. Covalent attachment of polyethylene glycol (PEG) derivatives on the capsule of pancreatic islets in aqueous media under moderate conditions is an example. This study aimed to find out whether higher polymer concentration could protect PEGylated islets from immune systems more efficiently without affecting their viability and morphology. Methoxy polyethylene glycol succinimidyl carbonate (mPEG-SC) with molecular weight of 5 kDa at different concentrations was grafted onto islets capsules. It was found that at polymer concentrations higher than 22mg/mL, islets lost their viability with changes in their surface structure as compared to free islets. At mPEG-SC concentrations less than 22 mg/mL, there was no change in morphology and viability of PEGylated islets, but PEGylation of islets at polymer concentration 7mg/mL was not sufficient for their camouflaging. Polymer concentration of 22 mg/mL was more effective in weakening the immunogenicity of islets. It was concluded that increasing the 5kDa mPEG-SC concentration up to 22mg/ml protected islets from the immune cells more efficiently without affecting their viability and functionality.
机译：用特殊选择的聚合物修饰活细胞的表面是一种保护其免受免疫系统侵害的新方法。聚乙二醇（PEG）衍生物在中等条件下在水性介质中在胰岛胶囊上的共价附着是一个例子。这项研究旨在发现更高的聚合物浓度是否可以更有效地保护PEG化胰岛免受免疫系统影响，而又不影响其生存能力和形态。将具有不同浓度的5 kDa分子量的甲氧基聚乙二醇琥珀酰亚胺碳酸酯（mPEG-SC）移植到胰岛胶囊上。发现与游离胰岛相比，当聚合物浓度高于22mg / mL时，胰岛的表面结构发生变化，从而失去了生存能力。当mPEG-SC浓度低于22 mg / mL时，聚乙二醇化的胰岛的形态和生存能力没有变化，但是当聚合物浓度为7mg / mL时，胰岛的聚乙二醇化不足以掩饰它们。 22 mg / mL的聚合物浓度在减弱胰岛的免疫原性方面更有效。结论是将5kDa mPEG-SC浓度提高到22mg / ml可以更有效地保护胰岛免受免疫细胞的侵害，而不会影响它们的生存能力和功能。

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