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首页> 外文期刊>Artificial cells, blood substitutes, and biotechnology >Bone marrow stromal cell line promotes the proliferation of mast cell progenitors derived from cord blood CD34+ cells under serum-free conditions with a combination of both cell-cell interaction and soluble factors.
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Bone marrow stromal cell line promotes the proliferation of mast cell progenitors derived from cord blood CD34+ cells under serum-free conditions with a combination of both cell-cell interaction and soluble factors.

机译:骨髓基质细胞系在无血清条件下结合细胞-细胞相互作用和可溶性因子,促进源自脐血CD34 +细胞的肥大细胞祖细胞的增殖。

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摘要

A higher production of functional mast cells (MCs) can be generated by co-culturing cord blood-derived CD34+ cells with a human bone marrow stromal cell line under serum-free conditions supplemented with stem cell factor and IL-6. We addressed the question of whether the higher proliferation of MCs in this co-culture system might be due to the higher proliferation of MC progenitors. The stromal cell line increased the cell numbers of MC progenitors derived from cord blood-derived CD34+ cells, in a combination of cell-cell interactions between stromal cells and CD34+ cells, and as yet unidentified soluble factors derived from stromal cells.
机译:通过在无血清条件下补充脐带血来源的CD34 +细胞与人骨髓基质细胞系,并补充干细胞因子和IL-6,可以产生更高功能的肥大细胞(MC)。我们解决了以下问题:在此共培养系统中MC的较高增殖是否可能是由于MC祖细胞的较高增殖所致。在基质细胞和CD34 +细胞之间的细胞-细胞相互作用以及至今尚未鉴定的源自基质细胞的可溶性因子的结合中,基质细胞系增加了源自脐带血来源CD34 +细胞的MC祖细胞的数量。

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