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首页> 外文期刊>Artificial Organs >Donation after cardiac death: dynamic graft reconditioning during or after ischemic preservation?
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Donation after cardiac death: dynamic graft reconditioning during or after ischemic preservation?

机译：心脏死亡后的捐赠：在缺血性保存期间或之后进行动态移植修复？

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The benefit of gaseous oxygenation during storage of liver grafts from donors after cardiac death should be investigated as applied either during the whole period of preservation or only for the last 2 h prior to reperfusion. Rat livers were explanted 30 min after cardiac arrest of the donor and cold-stored (CS) for 20 h. Some grafts were subjected to venous systemic oxygen persufflation (VSOP) either for 20 h or for only 2 h subsequent to 18 h of CS. Viability of the livers was assessed thereafter by warm reperfusion in vitro. Twenty hours VSOP and 18 h CS + 2 h VSOP prevented mitochondrial protein breakdown of mitochondrial heat shock protein 70 and promoted a significant and approximately twofold increase in hepatic oxygen consumption, bile production, and energetic recovery upon warm reperfusion. No differences were seen whether VSOP was performed for 20 h or for only 2 h prior to reperfusion. Both techniques significantly abrogated parenchymal enzyme loss (alanine aminotransferase, aspartate aminotransferase) upon reperfusion compared with simple 20 h CS. An increase in perfusate levels of the mitochondrial enzyme glutamate dehydrogenase was observed only in the 20 h VSOP group. In conclusion, viability of donation after cardiac death liver grafts can still be augmented, similarly to continuous aerobic storage, by only endischemic reconditioning, both protocols preventing initial mitochondrial dysfunction and subsequent tissue injury.
机译：应当在整个保存期间或仅在再灌注前的最后2小时内研究在心脏死亡后捐献者的肝脏移植物储存过程中气体氧合的益处。供体心脏骤停后30分钟将大鼠肝脏移出，并冷藏（CS）20 h。在CS持续18小时后，对某些移植物进行静脉全身性氧透化（VSOP）20小时或仅2小时。此后通过体外热再灌注评估肝脏的生存力。二十小时VSOP和18小时CS + 2小时VSOP阻止了线粒体热休克蛋白70的线粒体蛋白分解，并促进肝耗氧量，胆汁生成和热再灌注时的能量恢复显着增加约两倍。 VSOP是在再灌注前进行了20 h还是仅进行了2 h，均未见差异。与简单的20 h CS相比，这两种技术在再灌注后均能显着消除实质性酶的损失（丙氨酸转氨酶，天冬氨酸转氨酶）。仅在20小时VSOP组中观察到线粒体酶谷氨酸脱氢酶的灌注液水平增加。总之，仅通过局部缺血再造，类似于连续有氧存储，仍然可以增加心脏死亡肝移植后捐赠的生存力，这两种方法均可以防止最初的线粒体功能障碍和随后的组织损伤。

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