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首页> 外文期刊>Artificial Organs >Effects of low intensity pulsed ultrasound on rat Schwann cells metabolism.
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Effects of low intensity pulsed ultrasound on rat Schwann cells metabolism.

机译：低强度脉冲超声对大鼠雪旺细胞代谢的影响。

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摘要

The effects of low intensity pulsed ultrasound to tenocytes and osteocytes are well understood and applied clinically. However, its effects on cultured Schwann cells are still not well elucidated. This study was designed to elucidate the effects of low intensity pulsed ultrasound on cultured Schwann cells and their possible molecular mechanism. Schwann cells were harvested from sciatic nerves of 3-day-old Sprague-Dawley rats. Low intensity pulsed ultrasound stimulator (frequency: 1 MHz, duration: 2 min, duty cycle: 20%, total treatment time: 3 min) was applied to three different culture conditions: regular culture medium containing 0, 5, or 10% fetal bovine serum. The viability, damage, and differentiation of Schwann cells were examined; gene expression was also analyzed. In the presence of 0.3 W/cm(2) pulsed ultrasound stimulation, increases in cell viability and decreases in cell apoptosis were observed in the serum deprivation group; in this culture condition, interleukin-1, tumor necrosis factor-alpha, and protein zero genes expression were downregulated and Desert Hedgehog transcripts gene expression was upregulated. We concluded that intervention with low intensity pulsed ultrasound could promote Schwann cell proliferation, prevent cell death, and keep adequate phenotype presentation for peripheral nerve recovery. The low intensity pulsed ultrasound stimulation to an injured nerve site could be applied as early as possible especially when the microenvironment is almost serum-free to obtain the most benefit.
机译：低强度脉冲超声对肌腱细胞和骨细胞的影响已广为人知，并在临床上得到应用。然而，其对培养的雪旺氏细胞的影响仍未得到很好的阐明。本研究旨在阐明低强度脉冲超声对培养的雪旺细胞的作用及其可能的分子机制。雪旺氏细胞是从3天大的Sprague-Dawley大鼠的坐骨神经中收获的。将低强度脉冲超声刺激器（频率：1 MHz，持续时间：2分钟，占空比：20％，总处理时间：3分钟）应用于三种不同的培养条件：含有0％，5％或10％胎牛的常规培养基血清。检查了雪旺氏细胞的活力，损伤和分化。还分析了基因表达。在存在0.3 W / cm（2）脉冲超声刺激的情况下，在血清剥夺组中观察到细胞活力的增加和细胞凋亡的减少;在这种培养条件下，白介素-1，肿瘤坏死因子-α和蛋白零基因的表达下调，而沙漠刺猬转录本的基因表达上调。我们得出的结论是，低强度脉冲超声干预可以促进雪旺氏细胞增殖，防止细胞死亡，并保持足够的表型表现以供周围神经恢复。可以尽早对受伤的神经部位施加低强度脉冲超声刺激，尤其是在微环境几乎无血清的情况下，以获得最大的益处。

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