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首页> 外文期刊>Artificial Organs >The evaluation of leukocytes in response to the in vitro testing of ventricular assist devices.
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The evaluation of leukocytes in response to the in vitro testing of ventricular assist devices.

机译：评估心室辅助设备的体外测试对白细胞的反应。

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Infection is a clinically relevant adverse event in patients with ventricular assist device (VAD) support. The risk of infection could be linked to a reduced immune response resulting from damage to leukocytes during VAD support. The purpose of this study was to develop an understanding of leukocyte responses during the in vitro testing of VADs by analyzing the changes to their morphology and biochemistry. The VentrAssist implantable rotary blood pump (IRBP) and RotaFlow centrifugal pump (CP) were tested in vitro under constant hemodynamic conditions. Automated hematology analysis of samples collected regularly over 25-h tests was undertaken. A new flow cytometric assay was employed to measure biochemical alteration, necrosis (7-AAD) and morphological alteration (CD45 expression) of the circulating leukocytes during the pumping process. The results of hematology analysis show the total leukocyte number and subset counts decreased over the period of in vitro tests dependent on different blood pumps. The percentage of leukocytes damaged during 6-h tests was 40.8?±?5.7% for the VentrAssist IRBP, 17.6?±?5.4% for the RotaFlow CP, and 2.7?±?1.8% for the static control (all n?=?5). Flow cytometric monitoring of CD45 expression and forward/side scatter characteristics revealed leukocytes that were fragmented into smaller pieces (microparticles). Scanning electron microscopy and imaging flow cytometry were used to confirm this. Device developers could use these robust cellular assays to gain a better understanding of leukocyte-specific VAD performance.
机译：在心室辅助装置（VAD）支持下，感染是临床相关的不良事件。感染的风险可能与VAD支持期间白细胞受损导致的免疫反应降低有关。这项研究的目的是通过分析VADs的形态和生化变化来了解VADs的体外测试过程中的白细胞反应。 VentrAssist植入式旋转血泵（IRBP）和RotaFlow离心泵（CP）在恒定的血液动力学条件下进行了体外测试。对经过25小时测试定期采集的样品进行自动血液学分析。一种新的流式细胞术用于测量泵送过程中循环白细胞的生化改变，坏死（7-AAD）和形态改变（CD45表达）。血液学分析结果表明，在体外试验期间，取决于不同的血泵，总白细胞数和亚组数减少。在6小时的测试中，受损的白细胞百分数对于VentrAssist IRBP是40.8％±5.7％，对于RotaFlow CP是17.6％±5.4％，对于静态对照是2.7％±1.8％（全部n≥=？）。 5）。流式细胞术监测CD45的表达和前向/侧向散射特征显示白细胞被破碎成较小的碎片（微粒）。扫描电子显微镜和成像流式细胞仪用于证实这一点。设备开发人员可以使用这些强大的细胞分析方法来更好地了解白细胞特异性VAD性能。

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