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Activation of Basophils Is a New and Sensitive Marker of Biocompatibility in Hemodialysis

机译：嗜碱性粒细胞的激活是血液透析中生物相容性的新的敏感标志。

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The hemodialysis procedure involves contact between peripheral blood and the surface of dialyzer membranes, which may lead to alterations in the pathways of innate and adaptive immunity. We aimed to study the effect of blood-membrane interaction on human peripheral basophils and neutrophils in hemodialysis with high- and low-permeability polysulfone dialyzers. The surface expression of CD203c (basophil selection marker) and CD63 (activation marker) after activation by the bacterial peptide formyl-methionyl-leucyl-phenylalanine (fMLP) or anti-Fc epsilon receptor I (Fc epsilon RI) antibody and the absolute number of basophils was investigated before and after hemodialysis with each of the dialyzers. Moreover, the expression on neutrophils of CD11b, the CD11b active epitope, and CD88 was analyzed in the same groups of individuals. The expression of CD63 in basophils following activation by fMLP was significantly higher in the patient group compared with that in healthy controls, but no differences were observed after activation by anti-Fc epsilon RI. During the hemodialysis procedure, the low-flux membrane induced up-regulation of CD63 expression on basophils, while passage through the high-flux membrane did not significantly alter the responsiveness. In addition, the absolute number of basophils was unchanged after hemodialysis with either of the dialyzers and compared with healthy controls. We found no significant differences in the expression of the neutrophil activation markers (CD11b, the active epitope of CD11b, and CD88) comparing the two different dialyzers before and after dialysis and healthy controls. Together, these findings suggest that alterations in basophil activity may be a useful marker of membrane bioincompatibility in hemodialysis.
机译：血液透析程序涉及外周血与透析器膜表面之间的接触，这可能导致先天和适应性免疫途径的改变。我们旨在研究高渗和低渗聚砜透析器在血液透析中血膜相互作用对人外周嗜碱性粒细胞和中性粒细胞的影响。 CD203c（嗜碱性粒细胞选择标记）和CD63（活化标记）在被细菌肽甲酰基-甲硫酰基-亮氨酰-苯丙氨酸（fMLP）或抗Fcε受体I（Fc epsilon RI）抗体激活后的表面表达和在每个透析器的血液透析前后对嗜碱性粒细胞进行了研究。此外，在同一组个体中分析了CD11b，CD11b活性表位和CD88在嗜中性粒细胞上的表达。与健康对照组相比，fMLP激活后嗜碱性粒细胞中CD63的表达明显高于健康对照组，但抗FcεRI激活后未观察到差异。在血液透析过程中，低通量膜诱导嗜碱性粒细胞CD63表达的上调，而通过高通量膜则没有明显改变反应性。此外，在使用任一透析器进行血液透析后，嗜碱性粒细胞的绝对数量没有改变，并且与健康对照相比。我们发现在透析前后和健康对照组之间，两种不同的透析器相比，中性粒细胞激活标志物（CD11b，CD11b和CD88的活性表位）的表达没有显着差异。总之，这些发现表明嗜碱性粒细胞活性的改变可能是血液透析中膜生物不相容性的有用标志。

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