• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Artificial Organs >The effect of lyophilization on graft acceptance in experimental xenotransplantation using porcine cornea.
【24h】

The effect of lyophilization on graft acceptance in experimental xenotransplantation using porcine cornea.

机译：在猪角膜实验性异种移植中冻干对移植物接受的影响。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The immunogenicity of lyophilized porcine cornea is unknown. The purpose of this study was to examine the possibility of using lyophilized porcine cornea as a substrate for ocular surface reconstruction. A porcine cornea stromal button was freeze-dried and vacuum-packed. Lyophilized and fresh porcine corneas were examined histologically, and then implanted into intrastromal pockets in live rat corneas. Cytokine concentrations in plasma and protein extracts from the corneal buttons of rats were measured using the fluorokine multianalyte profiling assay, and histologic examination was performed. Immunoreactivity to the alpha-gal epitope was not found in lyophilized porcine corneas, whereas it was found in several keratocytes in fresh porcine corneas. The median survival time of rat corneas receiving lyophilized porcine transplants was 28.0 days, significantly longer than the 14.0-day survival of rat corneas that received fresh porcine transplants (P < 0.05). CD45RO(+) and CD68(+) cells were observed in rejected corneas, and interleukin-2 and interferon-gamma were elevated in rat plasma and corneal tissue. The lyophilized porcine corneal stroma, which is devoid of alpha-gal epitope, is less antigenic, and may be a useful biomaterial for ocular surface reconstruction and corneal collagen supplementation.
机译：冻干猪角膜的免疫原性尚不清楚。这项研究的目的是检查使用冻干猪角膜作为眼表重建基质的可能性。将猪角膜基质纽扣冷冻干燥并真空包装。对冻干的新鲜猪角膜进行组织学检查，然后将其植入活大鼠角膜的基质内袋中。使用氟因子多分析物谱分析法测量大鼠角膜纽扣血浆和蛋白质提取物中的细胞因子浓度，并进行组织学检查。在冻干的猪角膜中未发现对α-gal抗原决定簇的免疫反应性，而在新鲜的猪角膜中的一些角膜细胞中发现了免疫反应性。接受冻干猪移植的大鼠角膜的中位生存时间为28.0天，明显长于接受新鲜猪移植的大鼠角膜的14.0天生存时间（P <0.05）。在排斥的角膜中观察到CD45RO（+）和CD68（+）细胞，并且大鼠血浆和角膜组织中白介素2和干扰素-γ升高。不含α-gal表位的冻干猪角膜基质的抗原性较低，并且可能是用于眼表重建和角膜胶原蛋白补充的有用生物材料。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号