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首页> 外文期刊>Artificial Organs >Constraint Stress, Microstructural Characteristics, and Enhanced Mechanical Properties of a Special Fibroblast-embedded Collagen Construct.
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Constraint Stress, Microstructural Characteristics, and Enhanced Mechanical Properties of a Special Fibroblast-embedded Collagen Construct.

机译：特殊成纤维细胞包埋的胶原蛋白构建体的约束应力，微结构特征和增强的机械性能。

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Cell-contracted collagen gels could provide rejection-free biomaterials for tissue engineering, but their application is limited by relatively low mechanical strength. We developed a special type I collagen construct (based on embedded fibroblasts) that was formed into a gel thread by using two anchors to constrain gel contraction in one direction. Each gel thread contained 2 mg of type I collagen and 1.0 x 10(6) fibroblasts, and had an initial volume of 3 mL. After 9 days in culture, this preparation was transformed into a thread-like construct measuring 26 x 2.3 x 0.21 mm. Investigation of the microstructure showed that the collagen fibrils longitudinally between two cells had most aligned with the direction of the constraint stress and had assumed higher density than those in the freely contracted controls. During culturing, the constraint stress first increased then decreased, with implications for the nature of the interaction between the embedded cells and collagen matrix. Under uniaxial tensile testing, the ultimate stress and material modulus increased by factors of 6 and 16, respectively, compared with controls, while the maximal strain decreased by 590%. Compared with the similar constructs in the literature, the thread gel was fabricated by means of a novel mold configuration so that it contracted to thread shape much faster, and more importantly, the constraint force was firstly reported in this article. The improved mechanical properties show that the gel thread could be an effective biomaterial for such tissue engineering applications as the fabrication of blood vessels, ligaments, and tendon grafts.
机译：细胞收缩的胶原蛋白凝胶可为组织工程提供无排斥的生物材料，但其应用受到相对较低的机械强度的限制。我们开发了一种特殊的I型胶原蛋白构建体（基于嵌入的成纤维细胞），该结构通过使用两个锚点在一个方向上约束凝胶收缩而形成了凝胶线。每个凝胶线均包含2 mg I型胶原蛋白和1.0 x 10（6）成纤维细胞，初始体积为3 mL。培养9天后，将该制剂转化为尺寸为26×2.3×0.21mm的线状构建体。对微观结构的研究表明，两个细胞之间纵向的胶原纤维与约束应力的方向最一致，并且假定密度高于自由收缩的对照。在培养过程中，约束应力首先增加然后减小，这暗示了嵌入细胞与胶原基质之间相互作用的性质。在单轴拉伸试验中，与对照组相比，极限应力和材料模量分别增加了6和16倍，而最大应变降低了590％。与文献中的类似构造相比，螺纹凝胶是通过一种新颖的模具构造制成的，因此它可以更快地收缩成螺纹形状，更重要的是，本文首次报道了约束力。改善的机械性能表明，凝胶线对于诸如血管，韧带和肌腱移植物等组织工程应用而言可能是一种有效的生物材料。

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