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首页> 外文期刊>Artificial Organs >Mesenchymal Stem Cells Seeded on Cross-Linked and Noncross-Linked Acellular Porcine Dermal Scaffolds for Long-Term Full-Thickness Hernia Repair in a Small Animal Model
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Mesenchymal Stem Cells Seeded on Cross-Linked and Noncross-Linked Acellular Porcine Dermal Scaffolds for Long-Term Full-Thickness Hernia Repair in a Small Animal Model

机译：间充质干细胞接种在交联和非交联的脱细胞猪皮肤支架上的小动物模型中的长期全厚度疝气修复。

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Biological meshes are biomaterials consisting of extracellular matrix that are used in surgery particularly for hernia treatment, thoracic wall reconstruction, or silicone implant-based breast reconstruction. We hypothesized that combination of extracellular matrices with autologous mesenchymal stem cells used for hernia repair would result in increased vascularization and increased strength of incorporation. We cultured autologous adipose-derived stem cells harvested from the inguinal region of Wistar rats on cross-linked and noncross-linked porcine extracellular matrices. In 24 Wistar rats, a standardized 2×4cm fascial defect was created and repaired with either cross-linked or noncross-linked grafts enriched with stem cells. Non-MSC-enriched grafts were used as controls. The rats were sacrificed at 3 months of age. The specimens were examined for the strength of incorporation, vascularization, cell invasion, foreign body reaction, and capsule formation. Both materials showed cellular ingrowth and neovascularization. Comparison of both tested groups with the controls showed no significant differences in the capsule thickness, foreign body reaction, cellularization, or vascularization. The strength of incorporation of the stem cell-enriched cross-linked extracellular matrix specimens was higher than in acellular specimens, but this result was statistically nonsignificant. In the noncross-linked extracellular matrix, the strength of incorporation was significantly higher in the stem cell group than in the acellular group. Seeding of biological meshes with stem cells does not significantly contribute to their increased vascularization. In cross-linked materials, it does not ensure increased strength of incorporation, in contrast to noncross-linked materials. Owing to the fact that isolation and seeding of stem cells is a very complex procedure, we do not see sufficient benefits for its use in the clinical setting.
机译：生物网是由细胞外基质组成的生物材料，用于外科手术中，特别是用于疝气治疗，胸壁重建或基于硅树脂植入物的乳房重建。我们假设细胞外基质与用于疝气修复的自体间充质干细胞的结合将导致血管形成增加和合并强度增加。我们在交联的和非交联的猪细胞外基质上培养了从Wistar大鼠腹股沟区域收获的自体脂肪干细胞。在24只Wistar大鼠中，创建了标准的2×4cm筋膜缺损，并用富含干细胞的交联或非交联移植物修复。将未富集MSC的移植物用作对照。在3个月大时处死大鼠。检查样品的掺入强度，血管形成，细胞侵袭，异物反应和胶囊形成。两种材料均显示细胞向内生长和新血管形成。将两个测试组与对照组进行比较，结果表明胶囊厚度，异物反应，细胞化或血管化没有显着差异。富含干细胞的交联细胞外基质标本的掺入强度高于无细胞标本，但该结果在统计学上无统计学意义。在非交联的细胞外基质中，干细胞组的掺入强度明显高于无细胞组。用干细胞播种生物网并不会明显促进其血管化。在交联材料中，与非交联材料相比，它不能确保增加的结合强度。由于干细胞的分离和接种是一个非常复杂的过程，因此我们认为在临床环境中使用干细胞没有足够的益处。

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