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首页> 外文期刊>Artificial Organs >An in vivo autotransplant model of renal preservation: cold storage versus machine perfusion in the prevention of ischemia/reperfusion injury.
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An in vivo autotransplant model of renal preservation: cold storage versus machine perfusion in the prevention of ischemia/reperfusion injury.

机译：肾脏保存的体内自体移植模型：冷藏与机械灌注在预防缺血/再灌注损伤中的作用。

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摘要

There is increasing proof that organ preservation by machine perfusion is able to limit ischemia/reperfusion injury in kidney transplantation. This study was designed to compare the efficiency in hypothermic organ preservation by machine perfusion or cold storage in an animal model of kidney autotransplantation. Twelve pigs underwent left nephrectomy after warm ischemic time; the organs were preserved in machine perfusion (n = 6) or cold storage (n = 6) and then autotransplanted with immediate contralateral nephrectomy. The following parameters were compared between the two groups of animals: hematological and urine indexes of renal function, blood/gas analysis values, histological features, tissue adenosine-5'-triphosphate (ATP) content, perforin gene expression in kidney biopsies, and organ weight changes were compared before and after preservation. The amount of cellular ATP was significantly higher in organs preserved by machine perfusion; moreover, the study of apoptosis induction revealed an enhanced perforin expression in the kidneys, which underwent simple hypothermic preservation compared to the machine-preserved ones. Organ weight was significantly decreased after cold storage, but it remained quite stable for machine-perfused kidneys. The present model seems to suggest that organ preservation by hypothermic machine perfusion is able to better control cellular impairment in comparison with cold storage.
机译：越来越多的证据表明，通过机器灌注保存器官能够限制肾脏移植中的缺血/再灌注损伤。本研究旨在比较在肾脏自体移植动物模型中通过机器灌注或冷藏保存的低温器官保存效率。温暖的缺血时间后，对十二头猪进行左肾切除术;将器官保存在机器灌注（n = 6）或冷藏（n = 6）中，然后通过立即对侧肾切除术进行自体移植。在两组动物之间比较了以下参数：肾脏功能的血液学和尿液指标，血液/气体分析值，组织学特征，组织中5'-三磷酸腺苷（ATP）含量，肾脏活检组织中穿孔素基因表达和器官比较保存前后的体重变化。通过机器灌注保存的器官中细胞ATP的含量明显更高;此外，细胞凋亡诱导的研究揭示了肾穿孔素在肾脏中的表达增强，与机器保存的相比，其经过了简单的低温保存。冷藏后器官重量显着下降，但对于机器灌注的肾脏，器官重量却保持稳定。本模型似乎表明，与冷藏相比，通过低温机器灌注进行器官保存能够更好地控制细胞损伤。

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