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首页> 外文期刊>Artificial Organs >Effects of cyclic stretch waveform on endothelial cell morphology using fractal analysis.
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Effects of cyclic stretch waveform on endothelial cell morphology using fractal analysis.

机译：使用分形分析的循环拉伸波形对内皮细胞形态的影响。

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摘要

Endothelial cells are remodeled when subjected to cyclic loading. Previous in vitro studies have indicated that frequency, strain amplitude, and duration are determinants of endothelial cell morphology, when cells are subjected to cyclic strain. In addition to those parameters, the current study investigated the effects of strain waveform on morphology of cultured endothelial cells quantified by fractal and topological analyses. Cultured endothelial cells were subjected to cyclic stretch by a designed device, and cellular images before and after tests were obtained. Fractal and topological parameters were calculated by development of an image-processing code. Tests were performed for different load waveforms. Results indicated cellular alignment by application of cyclic stretch. By alteration of load waveform, statistically significant differences between cell morphology of test groups were observed. Such differences are more prominent when load cycles are elevated. The endothelial cell remodeling was optimized when the applied cyclic load waveform was similar to blood pressure waveform. Effects of load waveform on cell morphology are influenced by alterations in load amplitude and frequency. It is concluded that load waveform is a determinant of endothelial morphology in addition to amplitude and frequency, and such effect is elevated by increase of load cycles. Due to high correlation between fractal and topological analyses, it is recommended that fractal analysis can be used as a proper method for evaluation of alteration in cell morphology and tissue structure caused by application of external stimuli such as mechanical loading.
机译：当受到循环负荷时，内皮细胞被重塑。先前的体外研究表明，当细胞受到循环应变时，频率，应变幅度和持续时间是内皮细胞形态的决定因素。除了这些参数外，当前的研究还研究了应变波形对通过分形和拓扑分析量化的培养的内皮细胞形态的影响。通过设计的装置对培养的内皮细胞进行循环拉伸，获得测试前后的细胞图像。分形和拓扑参数是通过开发图像处理代码来计算的。针对不同的负载波形进行了测试。结果表明通过循环拉伸进行细胞排列。通过改变负荷波形，观察到测试组细胞形态之间的统计学显着差异。当增加负载循环时，这种差异更加明显。当施加的循环负荷波形与血压波形相似时，可优化内皮细胞重塑。负载波形对细胞形态的影响受负载幅度和频率变化的影响。结论是，负载波形除了决定幅度和频率外，还决定着内皮的形态，并且随着负载周期的增加，这种影响会增强。由于分形和拓扑分析之间存在高度相关性，因此建议将分形分析用作评估由于施加外部刺激（例如机械负荷）而引起的细胞形态和组织结构变化的适当方法。

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