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首页> 外文期刊>Artificial Organs >Effects of Nano-hydroxyapatite/Poly(DL-lactic-co-glycolic acid) Microsphere-Based Composite Scaffolds on Repair of Bone Defects: Evaluating the Role of Nano-hydroxyapatite Content
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Effects of Nano-hydroxyapatite/Poly(DL-lactic-co-glycolic acid) Microsphere-Based Composite Scaffolds on Repair of Bone Defects: Evaluating the Role of Nano-hydroxyapatite Content

机译：纳米羟基磷灰石/聚（DL-乳酸-乙醇酸）微球基复合支架对骨缺损修复的作用：评估纳米羟基磷灰石含量的作用

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The aim of the current study was to prepare microsphere-based composite scaffolds made of nano-hydroxyapatite (nHA)/poly (DL-lactic-co-glycolic acid) (PLGA) at different ratios and evaluate the effects of nHA on the characteristics of scaffolds for tissue engineering application. First, microsphere-based composite scaffolds made of two ratios of nHA/PLGA (nHA/PLGA=20/80 and nHA/PLGA=50/50) were prepared. Then, the effects of nHA on the wettability, mechanical strength, and degradation of scaffolds were investigated. Second, the biocompatibility and osteoinductivity were evaluated and compared by co-culture of scaffolds with bone marrow stromal stem cells (BMSCs). The results showed that the adhesion, proliferation, and osteogenic differentiation of BMSCs with nHA/PLGA (50/50) were better than those with nHA/PLGA (20/80). Finally, we implanted the scaffolds into femur bone defects in a rabbit model, then the capacity of guiding bone regeneration as well as the in vivo degradation were observed by micro-CT and histological examinations. After 4 weeks' implantation, there was no significant difference on the repair of bone defects. However, after 8 and 12 weeks' implantation, the nHA/PLGA (20/80) exhibited better bone formation than nHA/PLGA (50/50). These results suggested that a proper concentration of nHA in the nHA/PLGA composite should be taken into account when the composite scaffolds were prepared, which plays an important role in the biocompatibility, degradation rate and osteoconductivity.
机译：本研究的目的是制备不同比例的纳米羟基磷灰石（nHA）/聚（DL-乳酸-共乙醇酸）（PLGA）制成的微球基复合支架，并评估nHA对纳米颗粒特性的影响。用于组织工程的支架。首先，制备由两种比率的nHA / PLGA（nHA / PLGA ＝ 20/80和nHA / PLGA ＝ 50/50）制成的基于微球的复合支架。然后，研究了nHA对支架的润湿性，机械强度和降解的影响。其次，通过将支架与骨髓基质干细胞（BMSC）共培养，评估并比较了生物相容性和骨诱导性。结果表明，nHA / PLGA（50/50）的BMSCs的粘附，增殖和成骨分化优于nHA / PLGA（20/80）的BMSC。最后，我们将支架植入兔模型的股骨骨缺损中，然后通过显微CT和组织学检查观察到引导骨再生的能力以及体内降解的能力。植入4周后，骨缺损的修复无明显差异。然而，在植入8周和12周后，nHA / PLGA（20/80）的骨形成比nHA / PLGA（50/50）更好。这些结果表明，在制备复合支架时，应考虑nHA / PLGA复合材料中适当的nHA浓度，这在生物相容性，降解率和骨传导性中起着重要作用。

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