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首页> 外文期刊>Artificial Organs >Isolation of primary human hepatocytes after partial hepatectomy: criteria for identification of the most promising liver specimen.
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Isolation of primary human hepatocytes after partial hepatectomy: criteria for identification of the most promising liver specimen.

机译：肝部分切除后原代人肝细胞的分离：最有希望的肝脏标本鉴定的标准。

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摘要

Demands for primary human hepatocytes are continuously increasing, while supply is insufficient due to limited cell sources. To improve cell availability, the present study investigates the influence of donor liver characteristics on the outcome of hepatocyte isolation from surgically removed liver tissue (n = 50). Hepatocytes were isolated from liver specimens using a standardized two-step collagenase perfusion technique. The patient's sex, previous chemotherapy, or histopathology have shown no influence. Donor age significantly affected the isolation outcome, but was not found suitable for predicting cell yields. Preoperative blood parameters did not correlate with cell yield, although cell function was affected: total protein, albumin synthesis, and cell viability were significantly decreased for serum gamma-glutamyl-transferase (GGT) levels >60 U/L. Specimens from patients with benign diseases gave significantly higher cell yields than tissue removed due to secondary and primary tumors, respectively. The indication for surgery is a valuable basis for identifying the most yielding specimens. Hepatocytes from donors with high GGT levels appear to show reduced functional properties.
机译：对原代人肝细胞的需求持续增长，而由于细胞来源有限，供应不足。为了提高细胞利用率，本研究调查了供体肝脏特征对通过手术切除的肝组织分离肝细胞结果的影响（n = 50）。使用标准化的两步胶原酶灌注技术从肝脏标本中分离出肝细胞。患者的性别，先前的化学疗法或组织病理学未显示出影响。供体年龄显着影响分离结果，但发现不适合预测细胞产量。术前血液参数与细胞产量无关，尽管细胞功能受到影响：血清γ-谷氨酰转移酶（GGT）水平> 60 U / L时，总蛋白，白蛋白合成和细胞活力显着降低。良性疾病患者的标本比分别由于继发性和原发性肿瘤而切除的组织产生的细胞产量高得多。手术指征是鉴定产量最高的标本的宝贵依据。来自GGT水平高的供体的肝细胞似乎显示出降低的功能特性。

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