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首页> 外文期刊>Artificial Organs >Spongy polyethersulfone membrane for hepatocyte cultivation: studies on human hepatoma C3A cells.
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Spongy polyethersulfone membrane for hepatocyte cultivation: studies on human hepatoma C3A cells.

机译：用于肝细胞培养的海绵状聚醚砜膜：对人肝癌C3A细胞的研究。

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There are different types of membranes used for hepatocyte cultivation. In our studies, spongy polyethersulfone (PES) membranes were examined as a support for hepatic cell cultivation in vitro. The extended surface of the membranes allows to introduce a high cell number especially in three-dimensional gel structure. Scanning electron microscopy analysis indicated that C3A cells used in our experiments grew well on PES membranes forming microvilli characteristic for normal hepatocytes. Analysis of cell viability proved that spongy PES membrane is well tolerated by J774 macrophages and did not stimulate nitric oxide synthesis. Bile canalicular structures were observed in fluorescence microscopy after F-actin staining with tetramethyl rhodamine iso-thiocyanate (TRITC)-phalloidin. The C3A cells showed high affinity to the PES membranes and adhered to almost 90% during the initial 24 h of incubation. Albumin production increased during static culture from the value of 805.2 +/- 284.4 (ng/24 h/initial 10(6) cells) during the first days, to 2017.6 +/- 505.9 (ng/24 h/initial 10(6) cells) after 10 days of culture. In conclusion, the spongy PES membranes can be used as scaffold for hepatocyte cultivation, especially for the creation of three-dimensional environments.
机译：存在用于肝细胞培养的不同类型的膜。在我们的研究中，检查了海绵状聚醚砜（PES）膜作为体外肝细胞培养的支持物。膜的延伸表面允许引入大量细胞，尤其是在三维凝胶结构中。扫描电子显微镜分析表明，我们实验中使用的C3A细胞在PES膜上生长良好，形成了正常肝细胞特有的微绒毛。细胞活力的分析证明，J774巨噬细胞可很好地耐受海绵状PES膜，并且不会刺激一氧化氮的合成。用四甲基罗丹明异硫氰酸酯（TRITC）-鬼笔环肽对F-肌动蛋白染色后，在荧光显微镜下观察到胆管结构。 C3A细胞显示出对PES膜的高度亲和力，并且在孵育的最初24小时内粘附到几乎90％。静态培养期间白蛋白的产生从头几天的805.2 +/- 284.4（ng / 24 h /初始10（6）细胞）增加到2017.6 +/- 505.9（ng / 24 h /初始10（6）细胞）细胞）培养10天后。总之，海绵状PES膜可用作肝细胞培养的支架，尤其是在三维环境的创造中。

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