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首页> 外文期刊>Asian-Australasian Journal of Animal Sciences >Effect of the Addition of beta-Hydroxybutyrate to Chemically Defined Maturation Medium on the Nuclear Maturation, Sperm Penetration and Embryonic Development of Porcine Oocytes In vitro
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Effect of the Addition of beta-Hydroxybutyrate to Chemically Defined Maturation Medium on the Nuclear Maturation, Sperm Penetration and Embryonic Development of Porcine Oocytes In vitro

机译：在化学定义的成熟培养基中添加β-羟基丁酸酯对猪卵母细胞体外核成熟，精子穿透和胚胎发育的影响

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摘要

We investigated the effects of various concentrations of P-hydroxybutyrate (BHB, 0, 0.1, 1 and 10 mM), a ketone body, added to chemically-defined maturation medium with or without energy substrates (glucose, pyruvate and lactate) on nuclear maturation rates up to the metaphase stage of the second meiotic division (M-II stage). In addition, we also assessed the influence of BHB on glutathione content, sperm penetration rate and embryonic development up to the blastocyst stage of oocytes matured under the presence of these energy substrates. Nuclear maturation rates up to the M-II stage of oocytes matured with BHB in each concentration group did not show a significant increase compared with the control (0 mM) groups in both the presence and absence of energy substrates. Although glutathione contents were not significantly different in each BHB concentration group, the sperm penetration rate in the 1 mM BHB group was significantly higher (p < 0.05) and the embryonic development rate of oocytes up to the blastocyst stage was significantly lower (p < 0.05) than the respective values of the control groups. These results suggest that BHB added to a chemically-defined maturation medium may stimulate sperm penetration while inhibiting embryonic development of porcine oocytes.
机译：我们研究了添加到化学定义的成熟介质中（含或不含能量底物（葡萄糖，丙酮酸和乳酸））的各种浓度的酮体对羟基苯甲酸丁二醇酯（BHB，0、0.1、1和10 mM）对核成熟的影响。直到第二个减数分裂分裂的中期（M-II期）。此外，我们还评估了BHB对在这些能量底物存在下成熟的卵母细胞的胚泡阶段直至谷胱甘肽含量，精子穿透率和胚胎发育的影响。在存在和不存在能量底物的情况下，在每个浓度组中，用BHB成熟的卵母细胞直至M-II期的核成熟速率都没有显示出与对照组（0 mM）相比显着增加。尽管每个BHB浓度组中的谷胱甘肽含量没有显着差异，但1 mM BHB组的精子穿透率显着较高（p <0.05），直到胚泡期的卵母细胞的胚胎发育率均显着较低（p <0.05） ），而不是对照组的相应值。这些结果表明，将BHB添加到化学定义的成熟培养基中可以刺激精子渗透，同时抑制猪卵母细胞的胚胎发育。

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