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首页> 外文期刊>Asian Journal of Microbiology, Biotechnology and Environmental Science >OPTIMIZATION OF CULTURAL CONDITIONS FOR CELLULASE PRODUCTION BY CELLULOLYTIC FUNGAL ORGANISMS AS THEY RELATE TO RESIDUAL SUGAR YIELD IN BROTH MEDIUM
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OPTIMIZATION OF CULTURAL CONDITIONS FOR CELLULASE PRODUCTION BY CELLULOLYTIC FUNGAL ORGANISMS AS THEY RELATE TO RESIDUAL SUGAR YIELD IN BROTH MEDIUM

机译：与发酵液中残留糖产量相关的纤维素真菌生物生产纤维素酶的文化条件的优化

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One hundred and fifteen samples made of 42 (36.5%) rumen fluid, 36 (31.3%) cowdung and 37 (32.2%) soil samples were used for the study. Rumen liquor was collected by use of stomach tubes inserted into the mouths of cows and by suction, liquor was collected into prewarmed thermoflasks under continuous flushing with carbondioxide. Cowdung samples were obtained with the aid of sterile swab sticks. Soil samples were collected into sterile universal containers. All samples were obtained from abattoirs situated at three locations in Benin City, Nigeria. Samples were investigated for cellulolytic fungi by Filter Paper Yeast Mineral broth method. Cellulase production was assayed by Carboxymethyl cellulose broth method while residual sugar yield and other cellulolytic activities were determined by 3, 5-Dinitrosalicylic acid, Filter paper, Microcrystalline and Viscometric methods. Cellulolytic fungal organisms isolated included Trichoderma reesei, Rhizopus stolonifer, Penicillium iriensis, Aspergillus fumigates, Aspergillus flavus, Aspergillus niger, Rhizopus ch+-inensis, Penicillium oxalicium, Trichoderma virens, Fusarium solani and Geotrichum candidum. Eight (53.3%), 3 (20.0%) and 4 (26.7%) of these organisms were isolated from soil, rumen fluid and cowdung respectively. The highest cellulase producing isolate was Aspergillus fumigates and the least was Trichoderma reesei. Total cellulase yield by all organisms was 14.74 ±1.2 IU/mL. Individual and collective yield was high compared to reports of earlierstudies. Residual sugar was optimally secreted into broth medium at 40°C and pH 5 by all isolates. The lowest sugar yields were obtained at 50°C, pH 6. Cellulolytic indices (residual sugar yield, exoglucanase activity, endoglucanase activity, extracellular protein yield and percentage loss of viscosity of carboxymethyl cellulose) of all isolates peaked at 38 ± 0.7°C and pH 5 ± 0.2 although there was no significant difference in the individual recorded values (P>0.05). Optimal pH and temperature for individual isolates were slightly different i.e., 34°C and pH 4.5 were optimal for Aspergillus fumigatus. The significance and implications of these are highlighted.
机译：该研究使用了115个由42个（36.5％）瘤胃液，36个（31.3％）牛粪和37个（32.2％）土壤样品制成的样品。使用插入牛口的胃管收集瘤胃液，并通过抽吸将其收集到预热的保温瓶中，并用二氧化碳连续冲洗。 Cowdung样品是在无菌拭子棒的帮助下获得的。将土壤样品收集到无菌的通用容器中。所有样品均取自位于尼日利亚贝宁市三个地点的屠宰场。通过滤纸酵母矿物肉汤方法研究样品的纤维素分解真菌。通过羧甲基纤维素肉汤法测定纤维素酶的产生，而通过3，5-二硝基水杨酸，滤纸，微晶和粘度法测定残留的糖产率和其他纤维素分解活性。分离出的纤维素分解真菌生物包括里氏木霉，根霉，青霉，熏蒸曲霉，黄曲霉，黑曲霉，ch + -inensis，草酸青霉，绿木霉，罐头镰刀霉和土t。从土壤，瘤胃液和牛粪中分别分离出八种（53.3％），3种（20.0％）和4种（26.7％）。产生纤维素酶最高的分离株是熏蒸曲霉，而最少的是里氏木霉。所有生物的总纤维素酶产量为14.74±1.2 IU / mL。与早期研究的报告相比，个人和集体的产量都很高。所有分离物均能在40°C和pH 5的条件下将残糖最佳地分泌到肉汤培养基中。在50°C，pH 6时获得最低的糖产量。所有分离株的纤维素分解指数（残余糖产量，外切葡聚糖酶活性，内切葡聚糖酶活性，细胞外蛋白产量和羧甲基纤维素的粘度损失百分比）在38±0.7°C和pH 5±0.2，尽管各个记录值之间无显着差异（P> 0.05）。各个分离物的最佳pH和温度略有不同，即烟曲霉的最佳pH为34°C和pH 4.5。突出了这些的意义和含义。

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