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首页> 外文期刊>Breeding science >Inter-sectional hybrids obtained from reciprocal crosses between Begonia semperflorens (section Begonia) and B. 'Orange Rubra' (section Gaerdita x section Pritzelia).
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Inter-sectional hybrids obtained from reciprocal crosses between Begonia semperflorens (section Begonia) and B. 'Orange Rubra' (section Gaerdita x section Pritzelia).

机译：从双倍体秋海棠（秋海棠）和B.'Orange Rubra'（Gaerdita x普里策利亚）之间的杂交获得的节间杂种。

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摘要

Inter-sectional hybrids were successfully obtained by the reciprocal crosses between 11 cultivars (including 6 diploids and 5 tetraploids) of Begonia semperflorens (SS & SSSS genomes) and B. 'Orange Rubra' (RR genome) with the aid of in vitro culture of mature or immature seeds on MS medium containing 0.1 mg l-1 alpha -naphthylacetic acid, 0.1 mg l-1 6-benzyladenine, 10 mg l-1 gibberellic acid, 30 g l-1 sucrose and 2.5 g l-1 gellan gum. Embryo rescue as ovary culture with immature seeds 12th-16th day after pollination (DAP) generally gave higher efficiency of plantlet formation, but in some cross combinations, culture of mature seeds (30 DAP) resulted in higher yield of plantlets. Flow cytometric analysis revealed that they were consisted of the plants with various genomic combinations (RS, RR, RSS, RRS, RRSS and RRRRSS) as estimated by the DNA contents of both parents. Hybridity of these plants with various genomic combinations including RR was confirmed by random amplified polymorphic DNA analysis. These results suggested that unreduced gamete formation and spontaneous chromosome doubling were involved in the hybrid formation of various ploidy levels and genomic combinations. These hybrids showed various levels of intermediate traits between both parents according to the genomic compositions, and some of them had desirable characters of both parents.
机译：通过四季秋海棠（SS和SSSS基因组）和B.'Orange Rubra'（RR基因组）的11个品种（包括6个二倍体和5个四倍体）之间的相互杂交成功地获得了跨节杂种。在含有0.1 mg l -1 α-萘乙酸，0.1 mg l -1 6-苄基腺嘌呤，10 mg l -1 < / sup>赤霉素，30克 -1 蔗糖和2.5克 -1 吉兰糖胶。授粉后第12-16天（DAP）用未成熟种子的卵巢培养进行胚胎抢救通常可以提高植株形成的效率，但是在某些交叉组合中，成熟种子的培养（30 DAP）可以提高植株的产量。流式细胞仪分析表明，它们由具有不同基因组组合（RS，RR，RSS，RRS，RRSS和RRRSRS）的植物组成，这是通过父母双方的DNA含量估算得出的。通过随机扩增的多态性DNA分析证实了这些植物与包括RR在内的各种基因组组合的杂交性。这些结果表明未减少的配子形成和自发染色体倍增参与了各种倍性水平和基因组组合的杂种形成。这些杂种根据基因组组成在父母双方之间表现出不同水平的中间性状，其中一些具有父母双方所希望的特征。

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