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首页> 外文期刊>Breeding science >Persimmon breeding in Japan for pollination-constant non-astringent (PCNA) type with marker-assisted selection
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Persimmon breeding in Japan for pollination-constant non-astringent (PCNA) type with marker-assisted selection

机译:日本的柿子育种,采用标记辅助选择的恒定授粉不收敛(PCNA)类型

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摘要

Oriental persimmon (Diospyros kaki) originated in Eastern Asia, and many indigenous cultivars have been developed in China, Japan, and Korea. These cultivars are classified into four groups based on their natural astringency loss on the tree and seed formation: pollination-constant non-astringent (PCNA), pollination variant non-astringent (PVNA), pollination-constant astringent (PCA), and pollination-variant astringent (PVA). PCNA is the most desirable type because the fruit can be eaten without any postharvest treatment; therefore, one of the goals of our persimmon breeding programs is to release superior PCNA cultivars. The PCNA genotype is recessive to the other three non-PCNA genotypes, and PCNA-type F-1 offspring are obtained exclusively from crosses among PCNA genotypes. Moreover, the number of superior PCNA cross parents have been limited. In the late 1980s, inbreeding depression became obvious, especially in terms of fruit size, tree vigor, and productivity. To mitigate the inbreeding, a backcross program using PCNA [(non-PCNA x PCNA) x PCNA] was started in 1990. This process, however, was inefficient because only 15% of the offspring were PCNA, and all offspring had to be grown to the fruiting stage. Therefore, molecular markers linked to the PCNA locus were developed for discriminating PCNA offspring. A molecular marker linked to Chinese PCNA has also been developed.
机译:东方柿(Diospyros kaki)起源于东亚,在中国,日本和韩国已经开发出许多本土品种。根据其在树上的自然涩味损失和种子形成情况,这些品种可分为四类:授粉恒定非涩味(PCNA),授粉变种非涩味(PVNA),授粉不变涩味(PCA)和授粉变种收敛剂(PVA)。 PCNA是最理想的类型,因为无需任何收获后处理即可食用水果。因此,我们的柿子育种计划的目标之一是释放优良的PCNA品种。 PCNA基因型相对于其他三种非PCNA基因型是隐性的,而PCNA型F-1后代仅从PCNA基因型之间的杂交中获得。此外,上等PCNA交叉亲本的数量受到限制。在1980年代后期,近亲衰退变得明显,尤其是在果实大小,树木活力和生产力方面。为了减轻近亲繁殖,1990年开始使用PCNA [(非PCNA x PCNA)x PCNA]进行回交程序。但是,此过程效率低下,因为只有15%的后代是PCNA,并且所有后代都必须生长到结果阶段。因此,开发了与PCNA基因座相关的分子标记物以区分PCNA后代。还开发了与中国PCNA相关的分子标记。

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