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首页> 外文期刊>Breeding science >Microarray analysis of sink-source transition in rice leaf sheaths.
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Microarray analysis of sink-source transition in rice leaf sheaths.

机译：水稻叶鞘中库-源过渡的微阵列分析。

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摘要

In rice (Oryza sativa) plants, the leaf sheaths of the upper leaves accumulate a large amount of starch before heading, and the accumulated starch is converted to sucrose and translocated to the panicles after heading. To analyse the regulation of sink-source transition, a large-scale monitoring of gene expression by microarray analysis using 8987 rice expressed sequence tags (ESTs) was conducted. A total of 102 developmentally regulated genes in the leaf sheaths of the first leaves below the flag leaves during the heading period were identified. The identified genes included multiple genes related to starch biosynthesis, cell division and expansion, and photosynthesis. All genes showed early stage-preferential expression, probably reflecting the decrease of starch biosynthesis, end of elongation and decrease of photosynthesis in the leaf sheaths during the heading period, respectively. The expression patterns of the genes for starch biosynthesis enzymes and alpha -tubulin suggest that the leaf sheaths displayed both accumulating- and consuming-sink functions at the very early stage of the heading period. Northern blot analysis of the genes for starch degradation and sucrose biosynthesis enzymes ( alpha -amylase3D and sucrose phosphate synthase) revealed that the induction of the genes did not occur when the starch amount began to decrease, suggesting that the mechanisms of starch degradation and sucrose re-synthesis in the leaf sheaths during the heading period were different from those in germinating seeds. Furthermore, 18 developmentally regulated genes, whose functions in the leaf sheaths are unknown, were identified including 7 genes preferentially expressed in the bottom part of the leaf sheaths, implying that they were involved in functions related to the starch metabolism. The results obtained showed the effectiveness of the microarray technique to analyse complex and uncharacterized phenomena..
机译：在水稻（Oryza sativa）植物中，抽穗前上部叶片的叶鞘积累了大量淀粉，抽穗后积累的淀粉转化为蔗糖并转移到穗上。为了分析宿源过渡的调控，通过使用8987水稻表达序列标签（EST）的微阵列分析对基因表达进行了大规模监测。在抽穗期，在旗叶下面的第一片叶子的叶鞘中总共鉴定出102个发育调控基因。鉴定出的基因包括与淀粉生物合成，细胞分裂和扩增以及光合作用相关的多个基因。所有基因均显示出早期优先表达，可能分别反映了抽穗期叶片鞘内淀粉生物合成的减少，伸长的结束和光合作用的减少。淀粉生物合成酶和α-微管蛋白的基因表达模式表明，叶鞘在抽穗期的早期就显示出积累和消耗的功能。对淀粉降解和蔗糖生物合成酶（α-淀粉酶3D和蔗糖磷酸合酶）基因的Northern印迹分析表明，当淀粉量开始减少时，基因的诱导并没有发生，这表明淀粉降解和蔗糖的机制重新发生。抽穗期叶片鞘中的合成与发芽种子中的不同。此外，还鉴定出18个发育调控基因，其在叶鞘中的功能尚不清楚，其中包括优先在叶鞘底部表达的7个基因，这表明它们参与了与淀粉代谢相关的功能。获得的结果表明，微阵列技术可用于分析复杂的未表征现象。

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