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首页> 外文期刊>Breeding science >Expression and sequence analysis of systemic regulation gene for symbiosis, NTS1/GmNARK in supernodulating soybean cultivar, Sakukei 4.
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Expression and sequence analysis of systemic regulation gene for symbiosis, NTS1/GmNARK in supernodulating soybean cultivar, Sakukei 4.

机译：NTS1 / GmNARK共生系统调节基因在超结瘤大豆品种Sakukei 4中的表达及序列分析

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摘要

The nodulation programme is regulated systemically in leguminous hosts. This mechanism is referred to as the autoregulation of nodulation. Recently, a gene involved in the regulation has been isolated from the model legume Lotus japonicus (HAR1), soyabean (NTS1/GmNARK) and pea (PsSYM29) that encode a receptor-like kinase, similar to the well-characterized Arabidopsis thaliana CLAVATA1. Here, the sequencing and expression analysis of the NTS1/GmNARK gene and its homologue GmCLV1A in a soyabean supernodulating cultivar, Sakukei 4, which shows a high yielding ability, especially in fields with low nitrogen fertility, is reported. Sequencing of the NTS1/GmNARK gene showed that Sakukei 4 has a nonsense mutation (AAA to TAA) near the transmembrane domain as well as En6500 that is the paternal donor parent of Sakukei 4. This mutation is involved in the supernodulating phenotype in both lines En6500 and Sakukei 4. There were no mutations in the GmCLV1A gene, including a single intron between the wild-type, Enrei and both supernodulating lines. Transcripts of both genes were detected in rhizobium-inoculated or non-inoculated cotyledons (7 day-old), primary leaves (14-day-old), leaves (21-day-old) and leaves (14 days after inoculation) by non-quantitative RT-PCR analysis. In the nodules and leaf buds, including the shoot apical meristem where CLAVATA1 is expressed in A. thaliana, both transcripts were also detected. The NTS1/GmNARK and its homologue GmCLV1A were transcribed constitutively in all the examined organs, regardless of the presence of symbiosis, stage of growth and normal or mutant status. It is concluded that may be necessary for a leguminous host to initiate the autoregulation system immediately after germination..
机译：豆科宿主系统地调节了结瘤程序。这种机制称为结瘤的自动调节。最近，已从模型豆科植物日本莲（HAR1），大豆（NTS1 / GmNARK）和豌豆（PsSYM29）中分离出一种参与调控的基因，该基因编码受体样激酶，与特征明确的拟南芥CLAVATA1相似。在此，报道了在大豆超结节栽培品种Sakukei 4中NTS1 / GmNARK基因及其同源物GmCLV1A的测序和表达分析，该品种显示出高的生产能力，特别是在氮肥力低的领域。 NTS1 / GmNARK基因的测序表明，Sakukei 4在跨膜结构域附近有一个无意义的突变（AAA到TAA），而En6500是Sakukei 4的父代供体。该突变参与了En6500两系的超结型表型。和Sakukei 4. GmCLV1A基因没有突变，包括野生型，Enrei和两个超结瘤系之间的单个内含子。在非根瘤菌接种的或未接种的子叶（7天大），初生叶片（14天大），叶片（21天大）和叶片（接种后14天）中检测到了这两个基因的转录本。 -定量RT-PCR分析。在结节和叶芽中，包括在拟南芥中表达CLAVATA1的茎尖分生组织中，也检测到了两个转录本。 NTS1 / GmNARK及其同源物GmCLV1A在所有检查的器官中均组成型转录，无论是否存在共生，生长阶段以及正常或突变状态。结论是豆科植物宿主发芽后立即启动自动调节系统可能是必要的。

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