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首页> 外文期刊>Breeding science >Frequency of Spontaneous Polyploidization of Embryos Regenerated from Cultured Anthers or Microspores of Brassica rapa var.pekinensis L. and B. oleracea var. capitata L.
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Frequency of Spontaneous Polyploidization of Embryos Regenerated from Cultured Anthers or Microspores of Brassica rapa var.pekinensis L. and B. oleracea var. capitata L.

机译：从小白菜和油菜的花药或小孢子再生的胚自发多倍化的频率。卡皮塔塔湖

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摘要

Anther culture and microspore culture are effective to produce homozygous diploids, and they have been used in the breeding of various species (Keller et al. 1982, Maheshwari et al. 1982, Takahata 1997). In the production of homozygous diploids by anther culture or microspore culture, haploid chromosomes must be doubled by a method, such as colchicine treatment, which is troublesome, time-consuming and often inefficient. Haploids, diploids and occasionally polyploids regenerate spontaneously in anther culture or microspore culture (Keller et al. 1975, Takahata 1997). These spontaneous diploids are convenient for: breeding because they do not require chromosome doubling treatment. In Brassica crops, the proportion of haploids, diploids and polyploidsin regenerated plants varies with the genotype (Takahata 1997, Farnham 1998). It was also reported that the proportion differed between anther culture and micro-spore culture when a genotype of Brassica napus (Lichter et al, 1988) and a genotypes of B oleracea (Wang et al. 1999) were examined.
机译：花药培养和小孢子培养可有效地产生纯合二倍体，它们已用于各种物种的育种（Keller等，1982; Maheshwari等，1982; Takahata 1997）。在通过花药培养或小孢子培养产生纯合二倍体时，必须通过诸如秋水仙碱处理之类的方法将单倍体染色体加倍，这是麻烦的，耗时的并且通常是低效的。单倍体，二倍体以及偶尔的多倍体在花药培养或小孢子培养中自发再生（Keller等，1975; Takahata，1997）。这些自发的二倍体便于：繁殖，因为它们不需要染色体加倍处理。在芸苔属作物中，再生植物中单倍体，二倍体和多倍体的比例随基因型而变化（Takahata，1997; Farnham，1998）。也有报道说，当检查甘蓝型油菜的基因型（Lichter等，1988）和油菜B的基因型（Wang等，1999）时，花药培养和小孢子培养的比例不同。

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