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首页> 外文期刊>Biochimica et biophysica acta: international journal of biochemistry and biophysics >Tepidopterin, 1-O-(L-threo-biopterin-2'-yl)-beta-N-acetylglucosamine from Chlorobium tepidum.
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Tepidopterin, 1-O-(L-threo-biopterin-2'-yl)-beta-N-acetylglucosamine from Chlorobium tepidum.

机译：来自绿球藻的Tepidopterin 1-O-（50-threo-biopterin-2'-hydroxyphenyl）-beta-N-acetylglucosamine。

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摘要

A novel pterin compound, designated as tepidopterin, was detected from a thermophilic photosynthetic green sulphur bacterium, Chlorobium tepidum. The amount of tepidopterin inside the cell was estimated to be 2-5 micromol g(-1) dry weight of cell. This compound was purified through a high performance liquid chromatography using preparative DeltaPak C18 column. This compound was characterized by chromatographic behavior and by absorption and fluorescence properties. Its structure was determined to be 1-O-(L-threo-biopterin-2'-yl)-beta-N-acetylglucosamine by 1D- and 2D-NMR spectroscopy, mass spectrometry and CD. The relative amount of tetrahydrotepidopterin was estimated to be 96.7% inside the cell, that of dihydrotepidopterin 2.9%, and that of fully oxidized tepidopterin 0.4%. The amount of tepidopterin within the cell increased continuously until the beginning of the stationary phase of the cell growth.
机译：从嗜热的光合作用绿色硫细菌，Clorobium tepidum中检测到一种新的蝶呤化合物，称为蝶呤蝶呤。该细胞内部的蝶呤蝶呤的量估计为细胞干重2-5 micromol g（-1）。该化合物使用制备的DeltaPak C18柱通过高效液相色谱法纯化。该化合物的特征在于色谱行为以及吸收和荧光性质。通过1D-和2D-NMR光谱，质谱和CD确定其结构为1-O-（L-苏-生物蝶呤-2'-基）-β-N-乙酰氨基葡糖。据估计，细胞内四氢蝶呤的相对量为96.7％，二氢蝶呤的相对量为2.9％，完全氧化的蝶呤的相对量为0.4％。细胞内的蝶呤蝶呤的量持续增加，直到细胞生长进入稳定期。

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