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Application of principal component analysis for data process in metabonomic studies

机译：主成分分析在代谢组学研究数据处理中的应用

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Aim To investigate biochemical mechanism of treating hyperactivity syndrome with Complex Ucncaria-stem mixture (USM). Methods In mouse model with apomorphine, the biochemical mechanism was studied by metabonomics method to evaluate the ability of drug to improve and regulate neurotransmitter through the determination of three kinds of monoamine neurotransmitters dopamine (DA), noeradrenaline (NE) and 5-hydroxytryptamine (5-HT) in both brain and plasma by HPLC-ECD method. Principal component analysis (PCA) was performed using multivariate statistics package in Matlab 7.0.1 R14 SP1 (Math Works). Results Complex Ucncaria-stem decoction can increase plasma DA concentration to the level close to that of control group and it can reduce DA concentration in brain to the level close to that of control group. The results showed that USM could increase DA concentration; reduce 5-HT concentration in plasma and DA concentration in mouse brain tissue. All these indicated that USM could regulate the unbalance of monoamine neurotransmitter, thus treat hyperactivity syndrome. Conclusion All these indicated that the therapeutic effect of USM on hyperactivity syndrome was related to the biochemicals base on which it regulated the unbalance of monoamine neurotransmitter. PCA result suggests that the metabonomics method may play an important role in identifying markers for producing the therapy action and funding the action mechanism of the traditional Chinese medicines to be related with down-regulating DA and 5-HT levels in brain tissue.
机译：目的探讨复杂的Ucccaria-Stem混合物（USM）治疗多动症的生化机制。方法在阿朴吗啡小鼠模型中，通过测定3种单胺类神经递质多巴胺（DA），去甲肾上腺素（NE）和5-羟色胺（5），用代谢组学方法研究了药物改善和调节神经递质的能力。 -HT）在脑和血浆中均采用HPLC-ECD方法。主成分分析（PCA）使用Matlab 7.0.1 R14 SP1（Math Works）中的多元统计数据包进行。结果复方刺杆汤能使血浆DA浓度升高至与对照组接近的水平，使脑中DA浓度降低至与对照组接近的水平。结果表明，USM可以增加DA的浓度。降低血浆中的5-HT浓度和小鼠脑组织中的DA浓度。所有这些表明USM可以调节单胺神经递质的失衡，从而治疗多动症。结论所有这些表明USM对多动症的治疗作用与生化物质调节单胺类神经递质失衡有关。 PCA结果表明，代谢组学方法可能在识别产生治疗作用的标志物和资助中药的作用机制方面起重要作用，这与下调脑组织中的DA和5-HT水平有关。

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