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首页> 外文期刊>Annales de biologie clinique >A paperless autoimmunity laboratory: myth or reality?
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A paperless autoimmunity laboratory: myth or reality?

机译：无纸化自身免疫实验室：神话还是现实？

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摘要

Testing for antinuclear antibodies is the most frequently prescribed analysis for the diagnosis of rheumatic diseases. Indirect immunofluorescence remains the gold standard method for their detection despite the increasing use of alternative techniques. In order to standardize the manual microscopy reading, automated acquisition and interpretation systems have emerged. This publication enables us to present our method of interpretation and characterization of antinuclear antibodies based on a cascade of analyses and to share our everyday experience of the G Sight from Menarini. The positiveegative discrimination on Hep cells 2000 is correct in 85% of the cases. In most of the false negative results, it is a question of aspecific or low titers patterns, but a few cases of SSA speckled patterns of low titers demonstrated a probability index below 8. Regarding the pattern recognition, some types and mixed patterns are not properly recognized. Concerning the probability index correlated in some studies to final titer, the weak fluorescence of certain patterns and the random presence of artifacts that distort the index don't lead us to continue it in our dailypractice. In conclusion, automated reading systems facilitate the reporting of results and traceability of patterns but still require the expertise of a laboratory technologist for positiveegative discrimination and for pattern recognition.
机译：抗核抗体的检测是风湿性疾病诊断的最常用处方分析。尽管越来越多地使用替代技术，但间接免疫荧光仍然是检测它们的金标准方法。为了使手动显微镜读数标准化，已经出现了自动采集和解释系统。该出版物使我们能够基于一系列分析来介绍抗核抗体的解释和表征方法，并分享我们从梅纳里尼（Menarini）获得的G Sight的日常经验。在85％的病例中，对Hep细胞2000的阳性/阴性判别是正确的。在大多数假阴性结果中，这是一个非特异性或低滴度模式的问题，但少数SSA斑点的低滴度模式显示出概率指数低于8。关于模式识别，某些类型和混合模式不正确公认的。关于在某些研究中与最终效价相关的概率指数，某些模式的弱荧光和扭曲指数的伪像的随机存在不会导致我们在日常实践中继续使用该指数。总之，自动阅读系统有助于结果的报告和模式的可追溯性，但仍需要实验室技术人员的专业知识才能进行正/负歧视和模式识别。

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