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首页> 外文期刊>Australasian Plant Pathology >Bioindicators for Ralstonia solanacearum race 4: plants in the Zingiberaceae and Costaceae families
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Bioindicators for Ralstonia solanacearum race 4: plants in the Zingiberaceae and Costaceae families

机译：青枯雷尔氏菌种族4的生物指标：姜科和科科植物

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摘要

Ralstonia solanacearum (Rs) race 4 causes bacterial wilt of edible ginger (Zingiber officinale) and limits crop production in the tropics. The use of bioindicator plants was investigated as a method for determining whether or not a field is contaminated with Rs race 4 before replanting with a susceptible crop. Tissue-cultured edible ginger (TCG), micro-sized red ginger (Alpinia purpurata; RG) and micro-sized spiral ginger (Costus barbatus; SG) were evaluated for suitability for detection of Rs race4. Candidate hosts were planted into the infested medium, and Rs populations in plant tissues, potting medium and drainage water were evaluated by viable plate counts and Immunostrips using a species-specific monoclonal antibody. TCG wilted completely within an average of 9 days at the highest (log 8 cfu/mL) level of inoculum, and within 19 days at the lowest (log 4 cfu/mL) inoculum level. RG wilted in 17-26 and SG in 29-39 days. The final populations of Rs race 4 in wilted TCG tissues, potting mediumand drainage water were between log 8.8-9.3 cfu/g, log 7.0-7.4 cfu/g and log 4.8-5.0 cfu/mL, respectively, regardless of the initial population levels. RG and SG did not enhance Rs population levels as well as TCG. On average, the bioindicator hosts increased the Rs populations by 100 to 1000-fold, thereby enabling detection with a simple immunodiagnostic test.
机译：青枯雷尔氏菌（Rsstonia solanacearum，Rs）第4类引起食用姜（Zingiber officinale）的细菌枯萎，并限制了热带地区的农作物产量。对生物指示剂植物的使用进行了研究，以作为在重新种植易感作物之前确定田地是否受到Rs第4族污染的方法。对组织培养的食用生姜（TCG），微型红姜（Alpinia purpurata; RG）和微型螺旋生姜（Costus barbatus; SG）进行Rs种族检测的适用性进行了评估4。将候选宿主种植到受感染的培养基中，并使用物种特异性单克隆抗体通过活板计数和免疫条评估植物组织，盆栽培养基和排水中的Rs种群。在最高（log 8 cfu / mL）接种物水平下，TCG在平均9天内完全枯萎，而在最低（log 4 cfu / mL）接种物水平下，在19天之内完全枯萎。 RG在17-26时枯萎，而SG在29-39天内枯萎。无论初始种群水平如何，在萎缩的TCG组织，盆栽培养基和排水中，Rs第4族的最终种群分别在log 8.8-9.3 cfu / g，log 7.0-7.4 cfu / g和log 4.8-5.0 cfu / mL之间。 。 RG和SG并没有提高Rs人口水平和TCG。平均而言，生物指示剂宿主使Rs种群增加了100到1000倍，从而可以通过简单的免疫诊断测试进行检测。

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