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Combined effects of organic matter and pH on acute toxicity of cadmium to Paronychiurus kimi (Collembola): Development of response surface model

机译:有机物和pH值对镉对Paronychiurus kimi(Collembola)急性毒性的联合影响:响应面模型的建立

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A second-order central composite design was employed to investigate the effect of organic matter (OM) content and soil acidity (pH) on the cadmium toxicity to Paronychiurus kimi (Lee), a collembolan species native to Korea. Two independent variables, OM and pH, were adjusted from 0 to 10% (in total dry wt) and 4.5 to 7.0, respectively. Cadmium concentrations tested were 10, 20, 40, 80, and 160mg/kg dry soil. The toxic effects that cadmium exerted on P. kimi (28-day median lethal concentration (LCs)) varied significantly with different combinations of OM content and pH. The second-order response surface model was appropriate for describing combined effects of OM and pH on the cadmium toxicity to P. kimi. Linear effects (OM and pH) were predominant contributors accounting for ''#x2248;70% of the total variance of cadmium toxicity to P. kimi. No significant quadratic (pHcpH) and interaction (OMcpH) effects were observed; therefore, the final second-order response surface model could be reduced as follows: 28-day LC of cadmium (mg/kg)=-94.87+26.69cOM+16.64cpH - 1.82cOMpo. Validation of the developed response surface model using 4 soil media with different OM content and pH resulted in a significant correlation coefficient (Rpo=0.97) between predicted and observed 28-day LC. Based on physico-chemical properties of the test media, developed models can be used to explain the variation of cadmium toxicities at various environmental conditions and may improve current environmental risk assessment procedures of metals.
机译:二阶中心复合设计用于研究有机物(OM)含量和土壤酸度(pH)对镉对Paronychiurus kimi(Lee)的毒性的影响。将两个独立变量OM和pH分别从0至10%(以总干重计)调整为4.5至7.0。所测试的镉浓度为10、20、40、80和160mg / kg干燥土壤。镉对P. kimi的毒性作用(28天平均致死浓度(LCs))随OM含量和pH的不同组合而显着不同。二级响应表面模型适用于描述OM和pH对镉对假单胞菌毒性的综合影响。线性效应(OM和pH)是主要因素,占``#x2248;镉对P. kimi毒性的总方差的70%''。没有观察到明显的二次(pHcpH)和相互作用(OMcpH)效应。因此,最终的二阶响应表面模型可以减少如下:镉的28天LC(mg / kg)=-94.87 + 26.69cOM + 16.64cpH-1.82cOMpo。使用4种具有不同OM含量和pH值的土壤介质对开发的响应面模型进行验证,结果导致预测的和观察到的28天LC之间具有显着的相关系数(Rpo = 0.97)。根据测试介质的理化性质,开发的模型可用于解释各种环境条件下镉毒性的变化,并可改善当前金属的环境风险评估程序。

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