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首页> 外文期刊>Auris, nasus, larynx >Expression of CC-chemokine receptor 7 (CCR7) and CXC-chemokine receptor 4 (CXCR4) in head and neck squamous cell carcinoma.
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Expression of CC-chemokine receptor 7 (CCR7) and CXC-chemokine receptor 4 (CXCR4) in head and neck squamous cell carcinoma.

机译：CC趋化因子受体7（CCR7）和CXC趋化因子受体4（CXCR4）在头颈部鳞状细胞癌中的表达。

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OBJECTIVES: We tried to clarify the correlation of the expression of CCR7 and CXCR4 with lymph node and distant metastasis. MATERIALS AND METHODS: We examined expression of CCR7 and CXCR4 in 9 HNSCC cell lines and 25 HNSCC tissues by semi-quantitative RT-PCR and immunohistochemistry study. We examined the expression levels of CCR7 and CXCR4 in undifferentiated and differentiated human normal keratinocyte. RESULTS: All cell lines expressed CCR7 mRNA, and three expressed CXCR4 mRNA. CCR7 and CXCR4 mRNAs were significantly higher in HNSCC tissues than in non-neoplastic tissues (p<0.05, respectively) and correlated with lymph node metastasis (p<0.05, respectively). The level of CXCR4 mRNA also correlated with distant metastasis (p<0.05). Immunohistochemistry demonstrated localization of CCR7 and CXCR4 to carcinoma cells and lymphocytes and immunohistochemical staining scores of CCR7 and CXCR4 also showed similar correlation to lymph node and distant metastasis with CCR7 and CXCR4 mRNA levels. The level of CCR7 mRNA was significantly higher in poorly and moderately differentiated than in well-differentiated HNSCC (p<0.05). The level of CCR7 mRNA in undifferentiated keratinocyte was significantly higher than that in differentiated keratinocyte. CONCLUSION: The expression of CCR7 in HNSCC increases by dedifferentiation and plays an important role in lymph node metastasis of HNSCC and CXCR4 plays an important role in lymph node metastasis as well as distant metastasis.
机译：目的：我们试图阐明CCR7和CXCR4的表达与淋巴结转移和远处转移的相关性。材料与方法：我们通过半定量RT-PCR和免疫组化研究，检测了9个HNSCC细胞系和25个HNSCC组织中CCR7和CXCR4的表达。我们检查了CCR7和CXCR4在未分化和分化的人类正常角质形成细胞中的表达水平。结果：所有细胞系均表达CCR7 mRNA，3种均表达CXCR4 mRNA。 HNSCC组织中的CCR7和CXCR4 mRNA显着高于非肿瘤组织（分别为p <0.05）和与淋巴结转移相关（分别为p <0.05）。 CXCR4 mRNA的水平也与远处转移相关（p <0.05）。免疫组化显示CCR7和CXCR4定位于癌细胞和淋巴细胞，并且CCR7和CXCR4的免疫组化染色评分也显示与淋巴结相似，CCR7和CXCR4 mRNA水平与远处转移有关。低分化和中分化水平的CCR7 mRNA水平明显高于分化良好的HNSCC（p <0.05）。未分化的角质形成细胞中CCR7 mRNA的水平显着高于分化的角质形成细胞中。结论：CCR7在HNSCC中的表达通过去分化而增加，在HNSCC的淋巴结转移中起重要作用，而CXCR4在淋巴结转移及远处转移中起重要作用。

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