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首页> 外文期刊>Biocontrol Science >A rapid and simple determination of food-borne Salmonella strains by using multi-channel oxygen electrodes.
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A rapid and simple determination of food-borne Salmonella strains by using multi-channel oxygen electrodes.

机译：使用多通道氧气电极可快速，简单地确定食源性沙门氏菌菌株。

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摘要

A rapid and simple procedure for the specific detection of Salmonella was developed by using a dissolved oxygen measurement device (DOX-96) with anti-Salmonella antibodies. In the DOX-96 system, a gold electrode is located at the bottom of each well, in a 96-hole plate. The gold electrode acts as the working electrode. The anti-Salmonella antibodies are then introduced into the system and immobilized on each well of the plate. Wells contained bound Salmonella typhimurium cells which were incubated at 37 degrees C, and the oxygen consumption in each well was monitored. It appeared that the oxygen consumption curve was inversely proportional to the growth of S. typhimurium. In the present method, S. typhimurium cells with an initial concentration of 2.5x100-2.5x108 CFU/ml in the sample showed an oxygen consumption curve within 13 h of incubation. Other microorganisms, such as Escherichia coli, Pseudomonas aeruginosa, Corynebacterium aquaticum and Bacillus subtilis did not interfere with the assay system. Thus the present method would be applicable toward a rapid and simple detection of Salmonella in food..
机译：通过使用带有抗沙门氏菌抗体的溶解氧测量装置（DOX-96），开发了一种快速简单的沙门氏菌特异性检测方法。在DOX-96系统中，金电极位于96孔板中每个孔的底部。金电极用作工作电极。然后将抗沙门氏菌抗体引入系统中，并固定在平板的每个孔中。孔含有结合的鼠伤寒沙门氏菌细胞，将其在37℃下孵育，并监测每个孔中的氧消耗。看来耗氧曲线与鼠伤寒沙门氏菌的生长成反比。在本方法中，样品中初始浓度为2.5x100-2.5x108 CFU / ml的鼠伤寒沙门氏菌细胞在孵育13小时内显示出耗氧曲线。其他微生物，例如大肠杆菌，铜绿假单胞菌，水产棒状杆菌和枯草芽孢杆菌均不会干扰测定系统。因此，本方法适用于食品中沙门氏菌的快速简单检测。

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