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首页> 外文期刊>Biocontrol Science >Rapid detection of shiga toxin-producing Escherichia coli in ground beef by an immunochromatography kit in combination with short-term enrichment and treatment for shiga toxin release.
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Rapid detection of shiga toxin-producing Escherichia coli in ground beef by an immunochromatography kit in combination with short-term enrichment and treatment for shiga toxin release.

机译：通过免疫色谱试剂盒结合短期富集和处理志贺毒素的释放，可快速检测碎牛肉中产生志贺毒素的大肠杆菌。

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摘要

To establish rapid methods to detect Shiga toxin (Stx)-producing Escherichia coli (STEC) in beef mince samples using an immunochromatography kit, results of 8-h enrichment in various types of broth with shaking were compared. In pure culture, Stx was detected in the culture of trypticase soy broth (TSB) at 42 degrees C and modified EC broth (mEC) at 36 degrees C from all or most serogroups of O26, O111, O128, O157 and OUT. Beef mince samples inoculated with each serogroup were enriched in TSB at 42 degrees C, mEC at 36 degrees C and mEC with novobiocin (NmEC) at 42 degrees C. Although all conditions led to the successful recovery of each serogroup using the plating method, enrichment in NmEC was relatively superior to other conditions in the detection of Stx using an immunochromatography kit. Results indicated that growth of STEC and release of Stx from cells were different in pure cultures and in culture with beef mince. In addition, polymyxin B treatment for 10 min at 37 degrees C and homogenization with glass beads enhanced detection of Stx. It is suggested that an immunochromatography kit in combination with enrichment in NmEC at 42 degrees C for 8 h, and treatment with polymyxin B or homogenizing, may be useful for the rapid detection of STEC contamination in beef mince.
机译：为了建立使用免疫色谱试剂盒检测牛肉碎肉样品中产生志贺毒素（Stx）的大肠杆菌（STEC）的快速方法，比较了在摇动下各种肉汤中8 h富集的结果。在纯培养物中，从O26，O111，O128，O157和OUT的所有或大多数血清组中，在42℃的胰蛋白酶酶大豆肉汤（TSB）和36℃的修饰的EC肉汤（mEC）的培养物中检测到Stx。分别将每个血清群接种的牛肉末样品在42°C的TSB，36°C的mEC和在42°C的Novobiocin（NmEC）的mEC中进行浓缩。尽管所有条件都导致使用接种方法成功恢复了每个血清群，但富集使用免疫层析试剂盒检测Stx时，NmEC中的HPLC相对优于其他条件。结果表明，在纯培养物中和在牛肉末中，STEC的生长和细胞中Stx的释放均不同。此外，多粘菌素B在37摄氏度下处理10分钟并用玻璃珠匀浆可增强Stx的检测。建议将免疫层析试剂盒与在42°C下浓缩NmEC 8小时并用多粘菌素B处理或均质化相结合，可用于快速检测牛肉碎肉中的STEC污染。

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