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首页> 外文期刊>Biocontrol Science >Colony hybridization method for rapid detection of Legionella spp.
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Colony hybridization method for rapid detection of Legionella spp.

机译：快速检测军团菌的菌落杂交方法。

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摘要

The colony hybridization (CH) method as a rapid method for detecting Legionella spp. was compared with the conventional culture method. In the comparison of colony count in which a suspension of L. pneumophila was examined, a positive correlation was shown between the results by both methods. The regression line was Y=0.85+1.34X and the correlation coefficient was 0.95. The maximum number of detectable colonies on the plate was 144 colony forming units, although there was a tendency for the CH data to give slightly lower counts than those of the culture method. The detection time in the CH method was half of that in the culture method, and the final result was obtained within 4 or 5 d. In addition the results by the CH method were rarely influenced by other contaminating microorganisms, since the transfer of colonies was performed at the initial stage of culture.
机译：菌落杂交（CH）法是一种快速检测军团菌的方法。与传统的培养方法进行了比较。在检查嗜肺乳杆菌的悬浮液的菌落计数的比较中，两种方法的结果之间显示出正相关。回归线为Y = 0.85 + 1.34X，相关系数为0.95。平板上可检测的最大菌落数为144个菌落形成单位，尽管CH数据的计数趋于比培养方法略低。 CH法的检测时间是培养法的一半，最终结果在4或5 d内获得。另外，由于菌落的转移是在培养的初始阶段进行的，因此CH方法的结果几乎不受其他污染微生物的影响。

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