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Determination of a favorable medium for detection of fungal extracellular protease

机译：确定用于检测真菌细胞外蛋白酶的有利培养基

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摘要

To determine a favorable medium for detecting fungal extracellular protease, a plate method was examined. In this study, 12 fungal strains were tested with 4 different types of Czapek Dox agar medium containing skim milk, and the clear zones around the colonies were compared on the different types of medium. Among them, 9 strains produced extracellular proteases. Triton X-100 was required to detect an extracellular protease from Alternaria alternata, while in case of Penicillium frequentans, saccharose was required. It was found that the media supplemented with both Triton X-100 and saccharose gave acceptable results in terms of extracellular protease detection.
机译：为了确定检测真菌细胞外蛋白酶的有利培养基，检查了平板法。在这项研究中，用含有脱脂乳的4种不同类型的Czapek Dox琼脂培养基测试了12种真菌菌株，并比较了不同类型培养基上菌落周围的透明区域。其中，有9个菌株产生细胞外蛋白酶。需要Triton X-100来检测交链孢霉的细胞外蛋白酶，而对于青霉菌，则需要蔗糖。发现在细胞外蛋白酶检测方面，同时补充有Triton X-100和蔗糖的培养基给出了可接受的结果。

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