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Isolation and characterization of cytochrome P450-producing bacteria from various environments

机译：各种环境中产生细胞色素P450的细菌的分离和表征

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Eight strains of cytochrome P450 (P450)-producing bacteria were isolated form M9 medium containing a P450-inducer as the sole carbon source from the environment. Strains EP1 to EP6 utilizing 2-ethoxyphenol as the sole carbon source were isolated from the soil of a weed-filled field, the water of a paddy field, laboratory effluent, domestic effluent and river water. Strain MP1 utilizing 2-methoxyphenol and strain CP1 utilizing camphor were isolated from oil-polluted soil and the soil of a weed-filled field, respectively. This suggests the distribution of P450-producing bacteria in the environment. Metyrapone (2-methyl-1,2-di-3-pyridyl-1-propanone) inhibited the growth of P450-producing bacteria on the media containing a P450-inducer as the sole carbon source, strongly suggesting that the P450 is involved in the catabolism of the inducer.
机译：从含有P450诱导剂作为唯一碳源的M9培养基中分离出八株产生细胞色素P450（P450）的细菌。从2-杂草丛田土壤，稻田水，实验室废水，生活污水和河水中分离出利用2-乙氧基苯酚作为唯一碳源的菌株EP1-EP6。分别从油污土壤和杂草田土壤中分离了利用2-甲氧基苯酚的MP1菌株和利用樟脑的CP1菌株。这表明环境中产生P450的细菌的分布。 Metyrapone（2-甲基-1,2-二-3-吡啶基-1-丙酮）在含有P450诱导剂作为唯一碳源的培养基上抑制了产生P450的细菌的生长，强烈表明P450参与了诱导剂的分解代谢。

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