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Isolation and characterization of a new cellulosome-producing Clostridium thermocellum strain

机译：一株新的产纤维素酶的热纤梭菌菌株的分离与鉴定

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摘要

The anaerobic thermophilic bacterium, Clostridium thermocellum, is a potent cellulolytic microorganism that produces large extracellular multienzyme complexes called cellulosomes. To isolate C. thermocellum organisms that possess effective cellulose-degrading ability, new thermophilic cellulolytic strains were screened from more than 800 samples obtained mainly from agriculture residues in Thailand using microcrystalline cellulose as a carbon source. A new strain, C. thermocellum S14, having high cellulose-degrading ability was isolated from bagasse paper sludge. Cellulosomes prepared from S14 demonstrated faster degradation of microcrystalline cellulose, and 3.4- and 5.6-fold greater Avicelase activity than those from C. thermocellum ATCC27405 and JW20 (ATCC31449), respectively. Scanning electron microscopic analysis showed that S14 had unique cell surface features with few protuberances in contrast to the type strains. In addition, the cellulosome of S14 was resistant to inhibition by cellobiose that is a major end product of cellulose hydrolysis. Saccharification tests conducted using rice straw soaked with sodium hydroxide indicated the cellulosome of S14 released approximately 1.5-fold more total sugars compared to that of ATCC27405. This newly isolated S14 strain has the potential as an enzyme resource for effective lignocellulose degradation.
机译：厌氧嗜热细菌梭状芽胞杆菌（Clostridium thermocellum）是一种强力的纤维素分解微生物，可产生称为纤维素小体的大型细胞外多酶复合物。为了分离具有有效的纤维素降解能力的嗜热梭状芽孢杆菌生物，使用微晶纤维素作为碳源，从主要来自泰国农业残留物的800多个样品中筛选出新的嗜热纤维素分解菌株。从蔗渣渣中分离出具有高纤维素降解能力的新菌株热纤梭菌S14。与分别来自热纤梭菌ATCC27405和JW20（ATCC31449）的纤维素相比，由S14制备的纤维素微粒表现出更快的微晶纤维素降解，以及Avicelase活性分别高3.4和5.6倍。扫描电子显微镜分析表明，S14具有独特的细胞表面特征，与典型菌株相比，具有很少的突起。另外，S14的纤维素体对作为纤维素水解的主要终产物的纤维二糖具有抑制作用。使用浸有氢氧化钠的稻草进行的糖化测试表明，与ATCC27405相比，S14的纤维素释放的总糖量高出约1.5倍。这种新分离的S14菌株具有作为酶资源有效降解木质纤维素的潜力。

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