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首页> 外文期刊>Biodegradation >Assessment of in-situ bioremediation at a refinery waste-contaminated site and an aviation gasoline contaminated site
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Assessment of in-situ bioremediation at a refinery waste-contaminated site and an aviation gasoline contaminated site

机译：评估炼油厂废物污染场地和航空汽油污染场地的原位生物修复

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摘要

A combination of geochemical, microbiological and isotopic methods were used to evaluate in-situ bioremediation of petroleum hydrocarbons at one site contaminated with refinery waste and a second site contaminated with aviation gasoline at Alameda Point, California. At each site, geochemical and microbiological characteristics from four locations in the contaminated zone were compared to those from two uncontaminated background locations. At both sites, the geochemical indicators of in-situ biodegradation included depleted soil gas and groundwater oxygen, elevated groundwater alkalinity, and elevated soil gas carbon dioxide and methane in the contaminated zone relative to the background. Radiocarbon content of methane and carbon dioxide measured in soil gas at both sites indicated that they were derived from hydrocarbon contaminant degradation. Direct microscopy of soil core samples using cell wall stains and activity stains, revealed elevated microbial numbers and enhanced microbial activities in contaminated areas relative to background areas, corroborating geochemical findings. While microbial plate counts and microcosm studies using soil core samples provided laboratory evidence for the presence of some microbial activity and contaminant degradation abilities, they did not correlate well with either contaminant location, geochemical, isotopic, or direct microscopy data.
机译：结合地球化学，微生物学和同位素方法，在加利福尼亚州阿拉米达点的一个站点被石油烃污染的现场和第二个站点被航空汽油污染的现场评估石油烃的现场生物修复。在每个站点，将污染区四个位置的地球化学和微生物特征与两个未污染背景位置的地球化学和微生物特征进行了比较。在这两个地点，原位生物降解的地球化学指标包括土壤气体和地下水中的氧气枯竭，地下水碱度升高以及受污染地区相对于本底而言土壤气体中二氧化碳和甲烷含量升高。在两个站点的土壤气体中测得的甲烷和二氧化碳的放射性碳含量表明，它们的排放源于碳氢化合物污染物的降解。使用细胞壁污渍和活性污渍对土壤核心样品进行直接显微镜检查，发现受污染区域的微生物数量相对于背景区域有所增加，微生物活性增强，这证实了地球化学的发现。尽管使用土壤核心样品进行的微生物板计数和微观研究为存在某些微生物活性和污染物降解能力提供了实验室证据，但它们与污染物的位置，地球化学，同位素或直接显微镜数据均没有很好的相关性。

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