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首页> 外文期刊>Asian Journal of Plant Sciences >Effect of explants density on the in vitro proliferation and growth of separated and cluster shoots of smooth cayenne pineapple (Ananas comosus L. Merr.).
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Effect of explants density on the in vitro proliferation and growth of separated and cluster shoots of smooth cayenne pineapple (Ananas comosus L. Merr.).

机译：外植体密度对光滑辣椒菠萝（ L. Merr。）离体和丛生芽的体外增殖和生长的影响。

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Multiplication rate was always used as the sole parameter for assessment of the optimal treatment for in vitro shoot formation. However, beside its effect on the shoot formation, different treatments had different impact on the production cost. Separated shoots and shoot cluster of different sizes (1, 2, 3 and 4 shoots per cluster) of Smooth cayenne pineapple were cultured at density of 1, 2, 3 and 4 separated shoots and 1 cluster on agar solidified full strength MS medium enriched with sucrose at 30 g L-1 and BAP at 2.25 mg L-1 for 2 months to investigate the effect of explants density and explant types on shoot formation per explant, total per liter and cost per shoot. Density of one separated shoot per culture resulted in the highest shoot formations per shoot (11 shoots) but lowest total shoot per liter (550 shoots) and highest cost per shoot (13 cents), while density of 4 shoots per culture resulted in lowest rate (4 shoots) but higher total per liter (1400 shoots) and lowest cost per shoot (10 cents). Cluster of two shoots had higher rate (7 shoots) and lower cost (16 cents) than cluster of 4 shoots (4 shoots and 20 cents) but both resulted in equal total shoot per liter (750 shoots). Separated shoot had higher shoot formation capacity, higher total per liter and lower cost per shoots than cluster of shoots and using of total per liter instead of rate per explant was the best parameter for selection of treatment of lower cost and higher shoots production.
机译：始终以繁殖率作为评估体外枝条形成最佳处理方法的唯一参数。但是，除了对芽形成的影响外，不同处理对生产成本的影响也不同。在琼脂固化的全强度MS培养基上以1,2,3,4分离芽和1簇的密度培养不同大小的光滑卡宴菠萝的芽和芽簇（每簇1,2、3和4芽）和1簇。以30 g L -1 的蔗糖和2.25 mg L -1 的BAP进行2个月的研究，研究外植体密度和外植体类型对每株外植体芽形成的影响，公升和每次拍摄成本。每种文化中一个单独的芽的密度导致每个芽的芽形成最高（11个芽），但每公升的最低芽总数（550个芽）和每次芽的成本最低（13美分），而每种培养物的4个芽的密度导致最低的发生率（4枝），但每升的总量更高（1400枝），单枝成本最低（10美分）。与4个新芽（4个新芽和20美分）相比，两个新芽的成簇（7个新芽）具有更高的成本（16美分），但成本却更低（16美分），但每公升的总新芽（750个新芽）均相等。分离的芽比成簇的芽具有更高的芽形成能力，更高的每升总产量和更低的每枝芽成本，并且以每升总含量代替每株外植体的比率是选择低成本和高芽产量的最佳处理参数。

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