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首页> 外文期刊>Biocontrol Science >A method combining immunomagnetic separation followed by plating and polymerase chain reaction assay for the detection of pathogenic Yersinia enterocolitica from food and fecal samples
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A method combining immunomagnetic separation followed by plating and polymerase chain reaction assay for the detection of pathogenic Yersinia enterocolitica from food and fecal samples

机译：结合免疫磁分离，平板接种和聚合酶链反应分析的方法，从食品和粪便样品中检测病原性小肠结肠炎耶尔森氏菌

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摘要

The present study was performed to evaluate the efficiency of a method combining immunomagnetic separation (IMS) followed by CIN agar plating and polymerase chain reaction (PCR) for the rapid, specific and sensitive detection of pathogenic Yersinia enterocolitica. The immunomagnetic beads for Y. enterocolitica O3 or O8, prepared in this study, could sensitively and specifically capture serovar O3 or O8. Furthermore, all of pathogenic Y. enterocolitica strains could be detected by PCR with a chromosomal gene (ail) as the target. The proposed method of combining IMS-plating and PCR with ail-primer set was shown to be capable of specifically and sensitively isolating and identifying as little as 10cfu/g of pathogenic Y. enterocolitica in food and human materials within 1-2 days. In addition, the proposed method has the advantage that the bacterial colonies as living cells in the samples are available for investigating the foodborne infections.
机译：进行本研究是为了评估快速，特异和灵敏地检测病原性小肠结肠炎耶尔森氏菌的方法，结合免疫磁分离（IMS），CIN琼脂平板接种和聚合酶链反应（PCR）的方法的效率。在这项研究中制备的小肠结肠炎耶尔森氏菌O3或O8的免疫磁珠可以敏感，特异性地捕获血清型O3或O8。此外，所有致病性小肠结肠炎耶尔森氏菌菌株都可以通过以染色体基因（ail）为靶标的PCR进行检测。结果表明，将IMS-plating和PCR与ail-primer set结合使用的方法能够在1-2天内特异性，灵敏地分离和鉴定食品和人类材料中10cfu / g致病性小肠结肠炎耶尔森菌。另外，所提出的方法的优点在于，样品中作为活细胞的细菌菌落可用于调查食源性感染。

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