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The Biofilm Environment Offers a Possible Condition for Inducing the Competency of DNA Recipient Cells through Nutritional Starvation

机译:生物膜环境为通过营养饥饿诱导DNA受体细胞的能力提供了可能的条件

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Transformation phenomena occurring under conditions mimicking the biofilm environment were investigated using Escherichia coli IM302 (as DNA recipient cells) and Providencia sp. WW2 (as surrounding cells in the biofilm model). In the case of planktonic IM302 cells kept at 25 degree C, the transformation took place exclusively in the absence of organic nutrients (COD = 0), and was not substantially observed in the range of COD = 30-1500 mg O sub(2)/L. On the other hand, in the case of biofilm IM302 cells, the transformation occurred at relatively high levels under the examined conditions (temperature = 5 or 25 degree C and COD = 0-1500 mg O sub(2)/L). These results indicated that the competency of biofilm IM302 cells was induced even in the presence of organic nutrients owing to nutritional starvation caused by WW2 cells.
机译:使用大肠杆菌IM302(作为DNA受体细胞)和Providencia sp。研究了在模仿生物膜环境的条件下发生的转化现象。 WW2(作为生物膜模型中的周围细胞)。对于浮游生物IM302细胞保持在25摄氏度的情况,该转化仅在不存在有机营养物的情况下发生(COD = 0),并且在COD = 30-1500 mg O sub(2)的范围内基本上未观察到/升另一方面,对于生物膜IM302细胞,在检查条件下(温度= 5或25摄氏度,COD = 0-1500 mg O sub(2)/ L),转化发生在相对较高的水平。这些结果表明,由于WW2细胞引起的营养饥饿,即使在有机营养物存在的情况下,生物膜IM302细胞的能力也被诱导。

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