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Comparison of the Cytotoxicity of High-Level Disinfectants by the MTT Assay and Direct Contact Assay

机译：MTT法和直接接触法比较高水平消毒剂的细胞毒性

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摘要

Most critical instruments are not designed for heat sterilization and autoclaving. These items are usually treated with chemical agents such as peracetic acid (PAA), glutaraldehyde (GA) and ortho-phthalaldehyde (OPA). MTT assay is often used to evaluate the in vitro cytotoxicity of these chemical agents. In this study, disinfectants were allowed to come in direct contact with cells. Their cytotoxicity was evaluated based on cell viability and adhesive properties. The results obtained from the direct contact method were compared with those obtained from the conventional MTT assay wherein the disinfectants were added into a nutrient medium. It was found that the two methods yielded very different results, especially when aldehyde- and halogen-containing disinfectants were tested, and that toxicity may be underestimated in the MTT assay. Hence, it can be assumed that the direct contact assay is more accurate when evaluating the cytotoxicity of residual chemicals. It was also observed that the cytotoxicity of PAA was lower than that of GA and OPA.
机译：大多数关键仪器并非为加热灭菌和高压灭菌而设计。这些物品通常用化学试剂处理，例如过氧乙酸（PAA），戊二醛（GA）和邻苯二甲醛（OPA）。 MTT测定法常用于评估这些化学试剂的体外细胞毒性。在这项研究中，允许消毒剂与细胞直接接触。根据细胞活力和粘附特性评估它们的细胞毒性。将直接接触法获得的结果与常规MTT分析获得的结果进行比较，在常规MTT分析中，将消毒剂添加到营养培养基中。发现这两种方法产生了截然不同的结果，特别是在测试含醛和卤素的消毒剂时，MTT分析的毒性可能被低估了。因此，可以认为在评估残留化学物质的细胞毒性时，直接接触测定更为准确。还观察到PAA的细胞毒性低于GA和OPA。

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