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首页> 外文期刊>Biomedical Engineering: Applications, Basis and Communications >Relationship between cell function and initial cell seeding density of primary porcine chondrocytes in vitro
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Relationship between cell function and initial cell seeding density of primary porcine chondrocytes in vitro

机译：猪原代软骨细胞的细胞功能与初始细胞密度的关系

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摘要

Maintenance of differentiated functional phenotype within in vitro chondrocyte culture requires seeding at high densities with large numbers of cells. However, optimal cell seeding numbers and densities remain elusive due to multiple varying parameters and different methodologies utilized in previous studies. In the current study, we tried to investigate the relationship between cell seeding number and differentiated functional phenotype of in vitro cultured chondrocytes. Varying numbers of primary porcine chondrocytes (0.25, 2.5, 25 and 250 K) were seeded in 96 well-plates and cultured for 4 weeks. Cell proliferation, glycosaminoglycan (GAG) production and gene expression levels of Sox9, aggrecan, COL II and COL I were evaluated. The results showed that GAG content was high in the 0.25 and 25 K groups, gene expression of Sox9 was high in the 2.5, 25 and 250 K groups and expression of COL II was high in the 25 K group, whereas expression of COL I was low in the 0.25, 25 and 250 K groups. It is concluded that the seeding number and density of the 25 K (78 K cells/cm ~2) group achieved the optimal balance between functional phenotype of individual cells and the total ECM production for in vitro cultured chondrocytes.
机译：在体外软骨细胞培养中维持分化的功能表型需要以高密度接种大量细胞。然而，由于先前研究中使用了多个变化的参数和不同的方法，最佳的细胞接种数量和密度仍然难以捉摸。在当前的研究中，我们试图研究细胞接种数量与体外培养的软骨细胞分化功能表型之间的关系。将不同数量的原代猪软骨细胞（0.25、2.5、25和250 K）接种到96孔板中，培养4周。评估了细胞增殖，糖胺聚糖（GAG）的产生以及Sox9，聚集蛋白聚糖，COL II和COL I的基因表达水平。结果表明，在0.25和25 K组中GAG含量高，在2.5、25和250 K组中Sox9的基因表达高，在25 K组中COL II的表达高，而COL I的表达高。在0.25、25和250 K组中较低。结论是，25 K（78 K细胞/ cm〜2）组的播种数量和密度实现了单个细胞的功能表型与体外培养的软骨细胞总ECM产量之间的最佳平衡。

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