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首页> 外文期刊>Biomedical Engineering: Applications, Basis and Communications >EFFECT OF BASIC FIBROBLAST GROWTH FACTOR ON BEHAVIOR ABILITY AND NEURONS OF THE HIPPOCAMPAL CA3 AREA IN ALZHEIMER'S DISEASE MODEL RATS
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EFFECT OF BASIC FIBROBLAST GROWTH FACTOR ON BEHAVIOR ABILITY AND NEURONS OF THE HIPPOCAMPAL CA3 AREA IN ALZHEIMER'S DISEASE MODEL RATS

机译：碱性成纤维细胞生长因子对阿尔茨海默病模型大鼠行为能力和海马CA3区神经元的影响

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The study aimed to examine the behavior ability and morphological changes in neuron in the hippocampal CA3 area of Alzheimer's disease (AD) model rats induced by β-amyloid protein (Aβ1-42) and observe the potentiality of the neuroprotective effect of basic fibroblast growth factor (bFGF) on the AD model rats. A total of 70 Wistar rats were randomly divided into the normal control group, the AD model group and the bFGF treatment group. The AD model rats were established by microinjection of Aβ1-42 solution into right hippocampal CA1 area. The bFGF was injected into the abdominal cavity of rats in the bFGF treatment group, and identical volume physiological saline was given for the other two groups. The colorimetric method was used to detect the choline acetyltransferase (ChAT) and acetylcholinesterase (AchE) activity after the behavior capability was determined. Real time-PCR (RT-PCR) was used to evaluate the expression of VEGF mRNA of the rat hippocampal CA3 area. Caspase-3 immunopositive cells in the hippocampal CA3 area were observed under a light microscopy and quantitative analysis were performed by cell morphometric technique. The ultra-microstructure of the neurons was also observed by a transmission electron microscopy (TEM). The results indicated that compared with the AD model group, the learning and memory abilities of the bFGF treatment group were obviously improved and the ChAT activity significantly increased (p < 0.05), whereas the AChE activity, expression of VEGF mRNA and quantity of Caspase-3 immunopositive cells notably decreased (p < 0.05). Under TEM, the neurons in the hippocampal CA3 area of the normal control group had moderate electron density, rule nucleus, integrity perinuclear membrane, evenly distributed chromoplasm and abundant cell organelle, however the neurons of the AD model group showed severely damaged, exhibiting cell body pyknosis, irregular nuclear membranes concentrated, intracytoplasm content concentrated, decreased or unclear organelles. The neuronic pathological lesion of the bFGF treatment group had lessened than that of the AD model group; some of them had distinct neuronal structure and abundant cell organelle. BFGF could efficiently improve the behavior ability and decrease the pathological lesion of hippocampus of the AD model rats, which might promote the neuroprotective effect in the AD.
机译：这项研究旨在检查由β淀粉样蛋白（Aβ1-42）诱导的阿尔茨海默病（AD）模型大鼠海马CA3区神经元的行为能力和形态变化，并观察碱性成纤维细胞生长因子的神经保护作用的潜力。 （bFGF）对AD模型大鼠的作用。将70只Wistar大鼠随机分为正常对照组，AD模型组和bFGF治疗组。通过向右海马CA1区显微注射Aβ1-42溶液建立AD模型大鼠。将bFGF注射到bFGF治疗组的大鼠的腹腔中，另外两组给予相同体积的生理盐水。确定行为能力后，用比色法检测胆碱乙酰转移酶（ChAT）和乙酰胆碱酯酶（AchE）的活性。实时荧光定量PCR（RT-PCR）用于评估大鼠海马CA3区VEGF mRNA的表达。在光学显微镜下观察海马CA3区的Caspase-3免疫阳性细胞，并通过细胞形态学技术进行定量分析。还通过透射电子显微镜（TEM）观察到神经元的超微结构。结果表明，与AD模型组相比，bFGF治疗组的学习记忆能力明显改善，ChAT活性显着提高（p <0.05），而AChE活性，VEGF mRNA表达和Caspase-数量3个免疫阳性细胞明显减少（p <0.05）。透射电镜下，正常对照组海马CA3区神经元具有中等的电子密度，规则核，完整的核周膜，色质均匀分布和细胞器丰富，而AD模型组的神经元受损严重，表现出细胞体脊柱前凸，核膜不规则浓缩，胞浆内含量浓缩，细胞器减少或不清楚。 bFGF治疗组的神经病理病变较AD模型组减轻。其中一些具有明显的神经元结构和丰富的细胞器。 BFGF可以有效改善AD模型大鼠的行为能力，减少海马的病理损害，可能促进AD的神经保护作用。

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